SUN-080 Diminished Akt Activation and Interaction with 14-3-3ζ is Associated with Insulin Resistance in Cardiomyocytes of Metabolic Syndrome Rats

• Published in: Journal of the Endocrine Society 2019-04, Vol.3 (Supplement_1)
• Main Authors: Landa-Galván, Huguet, Ríos-Castro, Emmanuel, Romero-García, Tatiana, Rueda, Angélica, Olivares-Reyes, Jesús
• Format: Article
• Language: English
• Subjects: Cardiovascular Endocrinology
• ISSN: 2472-1972; 2472-1972
• DOI: 10.1210/js.2019-SUN-080

Metabolic syndrome (MetS) is a cluster of physiological and biochemical disorders that increases the risk of cardiovascular disease, being insulin resistance (IR) a pivotal condition for its development. Alterations such as metabolic inflexibility, changes in calcium dynamics, oxidative stress and impaired insulin signaling have been reported in the state of IR in the heart. Akt kinase activation plays a central role in insulin actions, leading to glucose uptake and its use as the primary energy source in the heart. Akt activity and subcellular localization are highly regulated by phosphorylation and by the interaction with other proteins. It has been reported that 14-3-3 proteins regulate insulin response and in particular the ζ isoform exerts a positive regulation on Akt activation. Therefore, we studied the changes in Akt activation and interaction with 14-3-3ζ, in response to insulin in cardiomyocytes from a MetS rat model, induced by the consumption of 30% sucrose in the drinking water during 4 months. MetS rats developed obesity (body weight in g: MetS: 592±12 vs 415±5 in control rats (C), P