Printing Therapeutic Proteins in 3D using Nanoengineered Bioink to Control and Direct Cell Migration

A nanoengineered bioink loaded with therapeutic proteins is designed to direct cell function in a 3D printed construct. The bioink is developed from a hydrolytically degradable polymer and 2D synthetic nanoparticle. The synthesis of poly(ethylene glycol)‐dithiothreitol (PEGDTT) via a Michael‐like st...

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Bibliographic Details
Published in:Advanced healthcare materials 2019-06, Vol.8 (11), p.e1801553-n/a
Main Authors: Peak, Charles W., Singh, Kanwar Abhay, Adlouni, Mu'ath, Chen, Jeffrey, Gaharwar, Akhilesh K.
Format: Article
Language:English
Subjects:
3D printing
Active control
additive manufacturing
Angiogenesis
Biological activity
Cell adhesion & migration
Cell migration
Chemical synthesis
Construction
Controlled release
Degradation
Dithiothreitol
hydrogel bioink
Inks
Kinetics
Mechanical properties
nanocomposites
Nanoparticles
Polyethylene glycol
Polymerization
protein therapeutics
Proteins
Regenerative medicine
Sustained release
Three dimensional printing
Tissue engineering
two‐dimensional (2D) nanomaterials
Umbilical vein
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Summary:A nanoengineered bioink loaded with therapeutic proteins is designed to direct cell function in a 3D printed construct. The bioink is developed from a hydrolytically degradable polymer and 2D synthetic nanoparticle. The synthesis of poly(ethylene glycol)‐dithiothreitol (PEGDTT) via a Michael‐like step growth polymerization results in acrylate terminated degradable macromer. The addition of 2D nanosilicates to PEGDTT results in formation of shear‐thinning bioinks with high printability and structural fidelity. The mechanical properties, swelling kinetics, and degradation rate of 3D printed constructs can be modulated by changing the ratio of PEG:PEGDTT and nanosilicates concentration. Due to high surface area and charged characteristic of nanosilicates, protein therapeutics can be sequestered in 3D printing structure for prolong duration. Sustained release of pro‐angiogenic therapeutics from 3D printed structure, promoted rapid migration of human endothelial umbilical vein cell. This approach to design biologically active inks to control and direct cell behavior can be used to engineer 3D complex tissue structure for regenerative medicine. A nanoengineered bioink loaded with therapeutic proteins is designed to direct cell function in a 3D printed construct. This approach to design biologically active inks to control and direct cell migration can be used to engineer 3D vascularized tissue structure for regenerative medicine.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201801553