Singlet Oxygen Sensor Green is not a Suitable Probe for 1O2 in the Presence of Ionizing Radiation

A great number of fluorescent probes have been developed for detecting singlet oxygen ( 1 O 2 ), which is considered to be one of the most effective reactive oxygen species (ROS), especially in clinical applications. The commercially available fluorescent probe Singlet Oxygen Sensor Green (SOSG) is...

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Bibliographic Details
Published in:Scientific reports 2019-06, Vol.9 (1), p.8393-8393, Article 8393
Main Authors: Liu, Huanhuan, Carter, Philippe J. H., Laan, Adrianus C., Eelkema, Rienk, Denkova, Antonia G.
Format: Article
Language:English
Subjects:
631/1647/1888/1493
639/638/11/874
Energy
Fluorescent indicators
Free radicals
Gamma rays
Humanities and Social Sciences
Hydroxyl radicals
Ionizing radiation
Light
multidisciplinary
Oxygen
Photodynamic therapy
Radiation therapy
Reactive oxygen species
Science
Science (multidisciplinary)
Sensors
Spectrum analysis
Therapeutic applications
X-rays
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Summary:A great number of fluorescent probes have been developed for detecting singlet oxygen ( 1 O 2 ), which is considered to be one of the most effective reactive oxygen species (ROS), especially in clinical applications. The commercially available fluorescent probe Singlet Oxygen Sensor Green (SOSG) is widely used due to its reported high selectivity to 1 O 2 . In this study, we carried out systemic experiments to determine the activation of SOSG in the presence of ionizing radiation. The results show that the SOSG probe exhibits a pronounced fluorescence increase as a function of radiation dose delivered by gamma-rays as well as X-rays, in conditions where the formation of singlet oxygen is not expected. Furthermore, scavenger tests indicate that hydroxyl radicals may be involved directly or indirectly in the activation process of SOSG although the exact mechanism remains unknown.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-44880-2