Protective Role of Peroxiredoxin I in Heat-Killed Staphylococcus Aureus -infected Mice

Staphylococcus aureus (S. aureus) is a major gram-positive pathogen, which can cause toxic and immunogenic injuries both in nosocomial and community-acquired infections. Peroxiredoxin (Prx) I plays crucial roles in cellular apoptosis, proliferation, and signal transduction as well as in immunoregula...

Full description

Saved in:
Bibliographic Details
Published in:In vivo (Athens) 2019-05, Vol.33 (3), p.749-755
Main Authors: Sun, Hu-Nan, Liu, Yue, Wang, Jian-Nan, Wang, Chuang, Liu, Ren, Kong, Ling-Zu, Zhen, Xing, Chandimali, Nisansala, Cui, Yu-Dong, Kim, Sun-Uk, Lee, Dong-Seok, Yu, Dae-Yeul, Kim, Ji-Su, Jeong, Dong Kee, Kwon, Taeho, Han, Ying-Hao
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biomarkers
Cytokines - metabolism
Disease Models, Animal
Gene Knockout Techniques
Liver - immunology
Liver - metabolism
Liver - pathology
Mice
Mortality
Peroxiredoxins - genetics
Peroxiredoxins - immunology
Staphylococcal Infections - immunology
Staphylococcal Infections - microbiology
Staphylococcal Infections - mortality
Staphylococcus aureus - genetics
Staphylococcus aureus - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Staphylococcus aureus (S. aureus) is a major gram-positive pathogen, which can cause toxic and immunogenic injuries both in nosocomial and community-acquired infections. Peroxiredoxin (Prx) I plays crucial roles in cellular apoptosis, proliferation, and signal transduction as well as in immunoregulation. The present study aimed to investigate whether Prx I protects mice from death caused by the heat-killed Staphylococcus aureus. In the present study, we challenged the wild-type and Prx I-deficient mice with heat-killed S. aureus (HKSA). The effects of Prx I were evaluated by a series of in vitro and in vivo experiments including western blot, Haematoxylin and Eosin staining, splenocyte analysis and cytokines analysis. Intra-peritoneal (ip) inoculation of HKSA resulted in increased mortality of Prx I-knockout (KO) mice with severe liver damage and highly populated spleens with lymphocytes. Furthermore, HKSA infections also bursted the production of both pro-inflammatory and anti-inflammatory serum cytokines in Prx I KO compared to wild-type mice. Enhanced mortality of S. aureus-infected mice with Prx I deficiency suggested that Prx I may protect against the infection-associated lethality of mice.
ISSN:0258-851X
1791-7549
DOI:10.21873/invivo.11535