Amelin: a 4.1-related spectrin-binding protein found in neuronal cell bodies and dendrites

An immunoreactive, structural, and functional analog of erythrocyte protein 4.1 is present in neuronal cell bodies and dendrites. Other investigators have described the isolation of a 4.1 analog in brain with structural characteristics suggesting that its identity was synapsin I, a neuronal phosphop...

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Bibliographic Details
Published in:The Journal of neuroscience 1987-12, Vol.7 (12), p.3907-3914
Main Authors: Krebs, KE, Zagon, IS, Goodman, SR
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Binding and carrier proteins
Biological and medical sciences
brain
Brain Chemistry
Carrier Proteins - analysis
Chemical Phenomena
Chemistry
Dendrites - analysis
Fundamental and applied biological sciences. Psychology
membrane proteins
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins - analysis
neurons
Neurons - analysis
Proteins
spectrin
Synapsins
synaptic vesicles
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Description
Summary:An immunoreactive, structural, and functional analog of erythrocyte protein 4.1 is present in neuronal cell bodies and dendrites. Other investigators have described the isolation of a 4.1 analog in brain with structural characteristics suggesting that its identity was synapsin I, a neuronal phosphoprotein localized in the presynaptic terminal in association with small synaptic vesicles. In this report we demonstrate that the cell body/dendritic form of brain protein 4.1, which we have named amelin, is distinct from that of synapsin I on the basis of subcellular localization, migration in 2-dimensional gel electrophoresis, and structural criteria. We also demonstrate that amelin, like synapsin I, can bind brain spectrin on nitrocellulose paper. Neither amelin nor synapsin I binds calmodulin, as determined by a blot binding assay. We hypothesize that there exists in brain a family of 4.1-related proteins with distinct subcellular localization and function.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.07-12-03907.1987