Quantitative Evaluation of Ultrasound-Assisted Extraction of 1,3-β-glucans from Dictyophora indusiata Using an Improved Fluorometric Assay

• Published in: Polymers 2019-05, Vol.11 (5), p.864
• Main Authors: Fu, Yuan, Lin, Shang, Lu, Min, Wei, Si-Yu, Zhou, Jia, Zhao, Li, Zhang, Qing, Lin, De-Rong, Liu, Yun-Tao, Chen, Hong, Qin, Wen, Wu, Ding-Tao
• Format: Article
• Language: English
• Subjects: Accuracy; Aniline; Assaying; Calibration; Cellulose; Mushrooms; Optimization; Polysaccharides; Quantitative analysis; Response surface methodology; Ultrasonic imaging; Water chemistry
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym11050864

In the present study, an improved fluorometric assay based on aniline blue dye for the specific, accurate, and rapid quantification of 1,3-β-glucans in edible mushrooms was developed and fully validated. Furthermore, the improved method was successfully applied for the quantitative evaluation of water soluble 1,3-β-glucans extracted from by ultrasound-assisted extraction (UAE) with response surface methodology. Results showed that the improved method exhibited high specificity, accuracy, precision, repeatability, and stability, as well as a wide calibration range of 10-600 µg/mL ( > 99.9%). The maximum extraction yields of water soluble 1,3-β-glucans (1.20%) and total polysaccharides (5.41%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (56%), ultrasound extraction time (15 min), and ratio of liquid to raw material (22 mL/g). The results suggest that the improved fluorometric assay has great potential to be used as a routine method for the quantitative evaluation of 1,3-β-glucans in edible mushrooms and that the UAE method is effective for the extraction of 1,3-β-glucans from edible mushrooms.