Effect of MEK6 Gene and Salt Treatment on Adipogenesis in MEK6 Over-expressed 3T3-L1 Cells (P21-070-19)

• Published in: Current developments in nutrition 2019-06, Vol.3 (Suppl 1), p.nzz041.P21-070-19, Article nzz041.P21-070-19
• Main Authors: Lee, Myoungsook, Kang, Songjoo, Sung, Soyoung, Lee, Minjee, Kim, Juhee, Park, Mi-Young
• Format: Article
• Language: English
• Subjects: Obesity
• ISSN: 2475-2991; 2475-2991
• DOI: 10.1093/cdn/nzz041.P21-070-19

Both obesity and obesogenic environments(OE) to induce the fat accumulation are being the risk factors of various metabolic diseases. By 6 year-cohort study, we found that MEK6 gene and salt intake were positively related with inducing obese as part of OE factors such as lifestyle, genes, and eating habits etc. Objectives of study are to find the effects of MEK6 gene and salt treatment on adipogenesis using MEK6 over-expressed 3T3-L1 cells. After transformation for MEK6 over-expressed 3T3-L1 with lipofectamine 3000 (Invitrogen, California, USA) was confirmed by PCR method, salt was treated as 50 mM in the form of NaCl without cytotoxicity (MTT assay). Cell differentiation and TG synthesis (Oil Red O; ORO & DAPI/Nile red staining), and protein expression (western blotting analysis) associated with adipogenesis genes (PPAR-γ, C/EBP-α & aP2) and adipocytokines (adiponectin & leptin) were performed. ELISA kits were used for estimating inflammatory factors such as TNF-α, IL-1β, IL-10, MCP-1, PAI-1 etc. Real-time oxygen consumption in alive cells was measured every 17 seconds for 100 minutes. (Mito-Xpress O2 consumption array kit) All analyses were performed using SAS9.1 software and p-values of