Analysis of in vitro glucose utilization in a circadian pacemaker model

An in vitro glucose utilization method, based upon 14C-2-deoxyglucose kinetics in brain slices, has been used to study circadian rhythms in hypothalamic slices containing the suprachiasmatic nucleus (SCN). Spontaneous SCN metabolic activity in vitro is similar to that observed in vivo with higher me...

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Bibliographic Details
Published in:The Journal of neuroscience 1992-06, Vol.12 (6), p.2015-2021
Main Authors: Newman, GC, Hospod, FE, Patlak, CS, Moore, RY
Format: Article
Language:English
Subjects:
Animals
Anterior Hypothalamic Nucleus - metabolism
Autoradiography
Biochemistry and metabolism
Biological and medical sciences
Central nervous system
Circadian Rhythm
Deoxyglucose - metabolism
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
In Vitro Techniques
Magnesium - pharmacology
Male
Rats
Rats, Inbred Strains
Suprachiasmatic Nucleus - metabolism
Tetrodotoxin - pharmacology
Vertebrates: nervous system and sense organs
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Summary:An in vitro glucose utilization method, based upon 14C-2-deoxyglucose kinetics in brain slices, has been used to study circadian rhythms in hypothalamic slices containing the suprachiasmatic nucleus (SCN). Spontaneous SCN metabolic activity in vitro is similar to that observed in vivo with higher metabolic rates in subjective daytime and lower rates during subjective night. However, in vitro SCN metabolic activity during late subjective day is above that seen when glucose utilization is measured in vivo, suggesting that an inhibitory influence normally active in vivo is lost during slice isolation. Incubation of slices containing SCN in the presence of TTX exposes a TTX-insensitive component of metabolic activity in early subjective day, supporting prior suggestions that glucose utilization by the circadian oscillator continues in the absence of Na(+)-dependent action potentials. Studies with high Mg2+ concentrations are consistent with the hypothesis that most metabolic activity above the basal level observed with the glucose utilization method is related to synaptic activity. Pharmacological studies of the SCN brain slice model with radiotracers offer potential for analysis of both circadian rhythmicity and neural regulation.
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.12-06-02015.1992