The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain

The beta-adrenergic receptor kinase (beta ARK) phosphorylates the agonist-occupied beta-adrenergic receptor to promote rapid receptor uncoupling from Gs, thereby attenuating adenylyl cyclase activity. Beta ARK-mediated receptor desensitization may reflect a general molecular mechanism operative on m...

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Bibliographic Details
Published in:The Journal of neuroscience 1992-10, Vol.12 (10), p.4045-4055
Main Authors: Arriza, JL, Dawson, TM, Simerly, RB, Martin, LJ, Caron, MG, Snyder, SH, Lefkowitz, RJ
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
beta-Adrenergic Receptor Kinases
Biological and medical sciences
Brain - metabolism
Brain - physiology
Brain Chemistry
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Cyclic AMP-Dependent Protein Kinases - analysis
Cyclic AMP-Dependent Protein Kinases - genetics
Cyclic AMP-Dependent Protein Kinases - metabolism
Fundamental and applied biological sciences. Psychology
GTP-Binding Proteins - metabolism
Molecular Sequence Data
Proteins - metabolism
Rats
Synapses - chemistry
Synapses - metabolism
Vertebrates: nervous system and sense organs
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Summary:The beta-adrenergic receptor kinase (beta ARK) phosphorylates the agonist-occupied beta-adrenergic receptor to promote rapid receptor uncoupling from Gs, thereby attenuating adenylyl cyclase activity. Beta ARK-mediated receptor desensitization may reflect a general molecular mechanism operative on many G-protein-coupled receptor systems and, particularly, synaptic neurotransmitter receptors. Two distinct cDNAs encoding beta ARK isozymes were isolated from rat brain and sequenced. The regional and cellular distributions of these two gene products, termed beta ARK1 and beta ARK2, were determined in brain by in situ hybridization and by immunohistochemistry at the light and electron microscopic levels. The beta ARK isozymes were found to be expressed primarily in neurons distributed throughout the CNS. Ultrastructurally, beta ARK1 and beta ARK2 immunoreactivities were present both in association with postsynaptic densities and, presynaptically, with axon terminals. The beta ARK isozymes have a regional and subcellular distribution consistent with a general role in the desensitization of synaptic receptors.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.12-10-04045.1992