A novel, abundant sodium channel expressed in neurons and glia

A novel, voltage-gated sodium channel cDNA, designated NaCh6, has been isolated from the rat central and peripheral nervous systems. RNase protection assays showed that NaCh6 is highly expressed in the brain, and NaCh6 mRNA is as abundant or more abundant than the mRNAs for previously identified rat...

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Bibliographic Details
Published in:The Journal of neuroscience 1995-05, Vol.15 (5), p.3231-3242
Main Authors: Schaller, KL, Krzemien, DM, Yarowsky, PJ, Krueger, BK, Caldwell, JH
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Brain - cytology
Brain - metabolism
DNA, Complementary
Gene Library
Glial Fibrillary Acidic Protein - analysis
In Situ Hybridization
Molecular Sequence Data
Neuroglia - cytology
Neuroglia - metabolism
Neurons - cytology
Neurons - metabolism
Oligonucleotide Probes
Polymerase Chain Reaction
Protein Structure, Secondary
Rats
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Sequence Homology, Amino Acid
Sodium Channels - biosynthesis
Sodium Channels - chemistry
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Summary:A novel, voltage-gated sodium channel cDNA, designated NaCh6, has been isolated from the rat central and peripheral nervous systems. RNase protection assays showed that NaCh6 is highly expressed in the brain, and NaCh6 mRNA is as abundant or more abundant than the mRNAs for previously identified rat brain sodium channels. In situ hybridization demonstrated that a wide variety of neurons express NaCh6, including motor neurons in the brainstem and spinal cord, cerebellar granule cells, and pyramidal and granule cells of the hippocampus. RT-PCR and/or in situ hybridization showed that astrocytes and Schwann cells express NaCh6. Thus, this sodium channel is broadly distributed throughout the nervous system and is shown to be expressed in both neurons and glial cells.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.15-05-03231.1995