In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure

Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular...

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Bibliographic Details
Published in:Scientific reports 2019-06, Vol.9 (1), p.8716-11, Article 8716
Main Authors: Foglia, Fabrizia, Hazael, Rachael, Meersman, Filip, Wilding, Martin C., Sakai, Victoria García, Rogers, Sarah, Bove, Livia E., Koza, Michael Marek, Moulin, Martine, Haertlein, Michael, Forsyth, V. Trevor, McMillan, Paul F.
Format: Article
Language:English
Subjects:
631/57/2268
639/766/747
Aquaporins
Aqueous solutions
Bacteria
Bacteriology
Biological Physics
Biological Transport
Cytoplasm
Cytoplasm - metabolism
Datasets
Diffusion
Electrolytes
High pressure
Humanities and Social Sciences
Hydrodynamics
Hydrogenation
Intracellular
Investigations
Kinetics
Laboratories
Life Sciences
Macromolecules
Microbiology and Parasitology
multidisciplinary
Neutron Diffraction - methods
Neutron scattering
Neutrons
NMR
Nuclear magnetic resonance
Physics
Pressure
Proteins
Rotational diffusion
Science
Science (multidisciplinary)
Shewanella - cytology
Shewanella - metabolism
Shewanella oneidensis
Transport processes
Viscosity
Water - metabolism
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Summary:Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water diffusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500 MPa using quasi-elastic neutron scattering (QENS). The intracellular diffusion exhibits a significantly greater slowdown (by −10–30%) and an increase in rotational relaxation times (+10–40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells affecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-44704-3