Differences in the constituent fiber types contribute to the intermuscular variation in the timing of the developmental synapse elimination

• Published in: Scientific reports 2019-06, Vol.9 (1), p.8694-13, Article 8694
• Main Author: Lee, Young il
• Format: Article
• Language: English
• Subjects: 13/51; 14/19; 631/378/2571/2577; 631/80; 64/60; Animals; Animals, Newborn; Axons - physiology; Humanities and Social Sciences; Influence; Kinases; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Motor Neurons - physiology; multidisciplinary; Muscle contraction; Muscle Contraction - physiology; Muscle Fibers, Skeletal - physiology; Muscles; Musculoskeletal system; Myofibrils - physiology; Nervous system; Neuroglia - physiology; Neuromuscular Junction - physiology; Neuromuscular junctions; Neuronal-glial interactions; Reptiles & amphibians; Science; Science (multidisciplinary); Synapse elimination; Synapses; Synapses - physiology; Synaptogenesis; Time Factors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-45090-6

The emergence of a mature nervous system requires a significant refinement of the synaptic connections initially formed during development. Redundant synaptic connections are removed in a process known as synapse elimination. Synapse elimination has been extensively studied at the rodent neuromuscular junction (NMJ). Although several axons initially converge onto each postsynaptic muscle fiber, all redundant inputs are removed during early postnatal development until a single motor neuron innervates each NMJ. Neuronal activity as well as synaptic glia influence the course of synapse elimination. It is, however, unclear whether target muscle fibers are more than naïve substrates in this process. I examined the influence of target myofiber contractile properties on synapse elimination. The timing of redundant input removal in muscles examined correlates strongly with their proportion of slow myofibers: muscles with more slow fibers undergo elimination more slowly. Moreover, this intermuscular difference in the timing of synapse elimination appears to result from local differences in the rate of elimination on fast versus slow myofibers. These results, therefore, imply that differences in the constituent fiber types help account for the variation in the timing of the developmental synapse elimination between muscles and show that the muscle plays a role in the process.