Enhanced Nitrogen Fixation in a glgX -Deficient Strain of Cyanothece sp. Strain ATCC 51142, a Unicellular Nitrogen-Fixing Cyanobacterium

• Published in: Applied and environmental microbiology 2019-04, Vol.85 (7)
• Main Authors: Liberton, Michelle, Bandyopadhyay, Anindita, Pakrasi, Himadri B
• Format: Article
• Language: English
• Subjects: Carbohydrate Metabolism - genetics; Carbohydrates; Carbon; Carbon cycle; Catabolism; Cyanobacteria; Cyanobacteria - genetics; Cyanobacteria - metabolism; Cyanothece - cytology; Cyanothece - genetics; Cyanothece - metabolism; Diurnal; Diurnal variations; Ecosystems; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes; Genes, Bacterial - genetics; Genetic engineering; Genetic modification; Genetic transformation; Genetics and Molecular Biology; Glycogen; Glycogen - genetics; Glycogen - metabolism; Glycogen Synthase - genetics; Glycogen Synthase - metabolism; Growth rate; Internal energy; Metabolic Networks and Pathways - genetics; Metabolism; Nitrogen; Nitrogen - metabolism; Nitrogen Fixation; Oxygen - metabolism; Photosynthesis; Polysaccharides; Prokaryotes; Storage; Strains (organisms)
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.02887-18

Cyanobacteria are oxygenic photosynthetic prokaryotes with important roles in the global carbon and nitrogen cycles. Unicellular nitrogen-fixing cyanobacteria are known to be ubiquitous, contributing to the nitrogen budget in diverse ecosystems. In the unicellular cyanobacterium sp. strain ATCC 51142, carbon assimilation and carbohydrate storage are crucial processes that occur as part of a robust diurnal cycle of photosynthesis and nitrogen fixation. During the light period, cells accumulate fixed carbon in glycogen granules to use as stored energy to power nitrogen fixation in the dark. These processes have not been thoroughly investigated, due to the lack of a genetic modification system in this organism. In bacterial glycogen metabolism, the gene encodes a debranching enzyme that functions in storage polysaccharide catabolism. To probe the consequences of modifying the cycle of glycogen accumulation and subsequent mobilization, we engineered a strain of 51142 in which the gene was genetically disrupted. We found that the Δ strain exhibited a higher growth rate than the wild-type strain and displayed a higher rate of nitrogen fixation. Glycogen accumulated to higher levels at the end of the light period in the Δ strain, compared to the wild-type strain. These data suggest that the larger glycogen pool maintained by the Δ mutant is able to fuel greater growth and nitrogen fixation ability. Cyanobacteria are oxygenic photosynthetic bacteria that are found in a wide variety of ecological environments, where they are important contributors to global carbon and nitrogen cycles. Genetic manipulation systems have been developed in a number of cyanobacterial strains, allowing both the interruption of endogenous genes and the introduction of new genes and entire pathways. However, unicellular diazotrophic cyanobacteria have been generally recalcitrant to genetic transformation. These cyanobacteria are becoming important model systems to study diurnally regulated processes. Strains of the genus have been characterized as displaying robust growth and high rates of nitrogen fixation. The significance of our study is in the establishment of a genetic modification system in a unicellular diazotrophic cyanobacterium, the demonstration of the interruption of the gene in sp. strain ATCC 51142, and the characterization of the increased nitrogen-fixing ability of this strain.