A TaqMan Probe-Based Real-Time PCR Assay for the Rapid Identification of the Emerging Multidrug-Resistant Pathogen Candida auris on the BD Max System

is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial bloodstream and deep wound infections causing a high mortality rate mainly in intensive care unit (ICU) patients. Laboratories currently rely on phenotypic testing using commercial automated systems for ident...

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Bibliographic Details
Published in:Journal of clinical microbiology 2019-07, Vol.57 (7)
Main Authors: Lima, Amorce, Widen, Raymond, Vestal, Grant, Uy, Dominic, Silbert, Suzane
Format: Article
Language:English
Subjects:
Mycology
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Summary:is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial bloodstream and deep wound infections causing a high mortality rate mainly in intensive care unit (ICU) patients. Laboratories currently rely on phenotypic testing using commercial automated systems for identification of yeasts; however, this technique has often led to misidentification of to other closely related species. We developed and validated a TaqMan-based real-time PCR assay on the BD Max platform targeting ribosomal DNA (rDNA) region nucleotide sequences to quickly and accurately test for infection from culture and clinical specimens. The assay is highly specific, reproducible, and sensitive, allowing detection of as low as 1 CFU per reaction within 3 h.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01604-18