Voltage-sensitive dye imaging of primary motor cortex activity produced by ventral tegmental area stimulation

• Published in: The Journal of neuroscience 2014-06, Vol.34 (26), p.8894-8903
• Main Authors: Kunori, Nobuo, Kajiwara, Riichi, Takashima, Ichiro
• Format: Article
• Language: English
• Subjects: Animals; Dopaminergic Neurons - drug effects; Dopaminergic Neurons - physiology; Male; Motor Cortex - drug effects; Motor Cortex - physiology; Neural Pathways - drug effects; Neural Pathways - physiology; Neurons - drug effects; Neurons - physiology; Oxidopamine - toxicity; Rats; Rats, Wistar; Ventral Tegmental Area - drug effects; Ventral Tegmental Area - physiology; Voltage-Sensitive Dye Imaging
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.5286-13.2014

The primary motor cortex (M1) receives dopaminergic projections from the ventral tegmental area (VTA) through the mesocortical dopamine pathway. However, few studies have focused on changes in M1 neuronal activity caused by VTA activation. To address this issue, we used voltage-sensitive dye imaging (VSD) to reveal the spatiotemporal dynamics of M1 activity induced by single-pulse stimulation of VTA in anesthetized rats. VSD imaging showed that brief electrical stimulation of unilateral VTA elicited a short-latency excitatory-inhibitory sequence of neuronal activity not only in the ipsilateral but also in the contralateral M1. The contralateral M1 response was not affected by pharmacological blockade of ipsilateral M1 activity, but it was completely abolished by corpus callosum transection. Although the VTA-evoked neuronal activity extended throughout the entire M1, we found the most prominent activity in the forelimb area of M1. The 6-OHDA-lesioned VTA failed to evoke M1 activity. Furthermore, both excitatory and inhibitory intact VTA-induced activity was entirely extinguished by blocking glutamate receptors in the target M1. When intracortical microstimulation of M1 was paired with VTA stimulation, the evoked forelimb muscle activity was facilitated or inhibited, depending on the interval between the two stimuli. These findings suggest that VTA neurons directly modulate the excitability of M1 neurons via fast glutamate signaling and, consequently, may control the last cortical stage of motor command processing.