Semen cryopreservation for the Mediterranean brown trout of the Biferno River (Molise-Italy): comparative study on the effects of basic extenders and cryoprotectants

• Published in: Scientific reports 2019-07, Vol.9 (1), p.9703-9, Article 9703
• Main Authors: Di Iorio, Michele, Esposito, Stefano, Rusco, Giusy, Roncarati, Alessandra, Miranda, Marsia, Gibertoni, Pier Paolo, Cerolini, Silvia, Iaffaldano, Nicolaia
• Format: Article
• Language: English
• Subjects: 14/34; 14/63; 631/601; 631/601/2722; Animals; Artificial insemination; Body Fluids - drug effects; Body Fluids - metabolism; Comparative studies; Cryopreservation; Cryopreservation - veterinary; Cryoprotective Agents - classification; Cryoprotective Agents - pharmacology; Cryoprotectors; Eggs; Fertilization; Freezing; Hatching; Humanities and Social Sciences; Italy; Male; Motility; multidisciplinary; Rivers; Salmo trutta; Science; Science (multidisciplinary); Semen; Semen - chemistry; Semen Analysis - veterinary; Semen Preservation - veterinary; Sperm Motility; Spermatozoa - drug effects; Spermatozoa - physiology; Trout
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-45006-4

This study was designed to optimize the semen freezing protocol of the native Mediterranean brown trout inhabiting the Molise rivers through two experiments: an in vitro analysis of the effects of two basic extenders combined with three cryoprotectants on post-thaw semen quality; and an in vivo test to assess the fertilization and hatching rate. Semen was diluted at a ratio of 1:3 in a freezing medium composed of a glucose extender (A) or mineral extender (B). Each basic component contained 10% dimethylsulfoxide, dimethylacetamide or methanol. The post-semen quality was evaluated considering motility, duration of motility, viability and DNA integrity. The basic extender and cryoprotectant were shown to have significant effects on these variables, and the best results were obtained using extender A or B combined with dimethylsulfoxide (P