Comparative transcriptome analysis of cultivated and wild seeds of Salvia hispanica (chia)

• Published in: Scientific reports 2019-07, Vol.9 (1), p.9761-11, Article 9761
• Main Authors: Peláez, Pablo, Orona-Tamayo, Domancar, Montes-Hernández, Salvador, Valverde, María Elena, Paredes-López, Octavio, Cibrián-Jaramillo, Angélica
• Format: Article
• Language: English
• Subjects: 38; 38/90; 45/91; 631/208/199; 631/208/212/2019; Computational Biology; Fatty acids; Functional foods & nutraceuticals; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genetic diversity; Genetic Variation; Glutelin; Growth conditions; High-Throughput Nucleotide Sequencing; Humanities and Social Sciences; Lipid metabolism; Microsatellite Repeats; Molecular Sequence Annotation; multidisciplinary; Phenotype; Phylogeny; Polymorphism, Genetic; Population genetics; Population studies; Salvia - genetics; Salvia - metabolism; Salvia columbariae; Salvia hispanica; Science; Science (multidisciplinary); Seeds; Seeds - genetics; Seeds - metabolism; Single-nucleotide polymorphism; Transcription; Transcriptome
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-45895-5

Salvia hispanica (chia) constituted an important crop for pre-Columbian civilizations and is considered a superfood for its rich content of essential fatty acids and proteins. In this study, we performed the first comprehensive comparative transcriptome analysis between seeds from cultivated varieties and from accessions collected from native wild populations in Mexico. From the 69,873 annotated transcripts assembled de novo , enriched functional categories and pathways revealed that the lipid metabolism was one of the most activated processes. Expression changes were detected among wild and cultivated groups and among growth conditions in transcripts responsible for triacylglycerol and fatty acid synthesis and degradation. We also quantified storage protein fractions that revealed variation concerning nutraceutical proteins such as albumin and glutelin. Genetic diversity estimated with 23,641 single nucleotide polymorphisms (SNPs) revealed that most of the variation remains in the wild populations, and that a wild-type cultivated variety is genetically related to wild accessions. Additionally, we reported 202 simple sequence repeat (SSRs) markers useful for population genetic studies. Overall, we provided transcript variation that can be used for breeding programs to further develop chia varieties with enhanced nutraceutical traits and tools to explore the genetic diversity and history of this rediscovered plant.