Postmitotic annulate lamellae assembly contributes to nuclear envelope reconstitution in daughter cells

• Published in: The Journal of biological chemistry 2019-07, Vol.294 (27), p.10383-10391
• Main Authors: Ren, He, Xin, Guangwei, Jia, Mingkang, Zhu, Shicong, Lin, Qiaoyu, Wang, Xiangyang, Jiang, Qing, Zhang, Chuanmao
• Format: Article
• Language: English
• Subjects: Accelerated Communications; AL pore complex (ALPC); Animals; annulate lamellae; cell cycle; chromatin; Cytoplasm - metabolism; Drosophila; Drosophila - growth & development; Drosophila - metabolism; Embryo, Nonmammalian - metabolism; Embryonic Development; endoplasmic reticulum (ER); Endoplasmic Reticulum - metabolism; HEK293 Cells; HeLa Cells; Humans; intracellular membrane; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Mitosis; nuclear envelope; Nuclear Envelope - metabolism; nuclear membrane; nuclear pore; Nuclear Pore - metabolism; Nuclear Pore Complex Proteins - genetics; Nuclear Pore Complex Proteins - metabolism; nucleoporin
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.AC119.008171

In higher eukaryotic cells, the nuclear envelope (NE) is composed of double nuclear membranes studded with nuclear pore complexes (NPCs) and undergoes dynamic disassembly and reassembly during the cell cycle. However, how the NE and NPC reassemble remains largely unclear. Here, using HeLa, HEK293, and Drosophila cells, along with immunofluorescence microscopy and transmission EM methods, we found that postmitotic annulate lamellae (AL) assembly contributes to NE and NPC assembly. We observed that the AL are parallel membrane-pair stacks and possess regularly spaced AL pore complexes (ALPCs) that are morphologically similar to the NPCs. We found that the AL assemble in the cytoplasm during mitotic exit simultaneously with NE re-formation in daughter cells. Then, the assembled AL either bound the decondensing chromatin to directly transform into the NE or bound and fused with the outer nuclear membrane to join the assembling NE. The AL did not colocalize with sheet and tubular endoplasmic reticulum (ER) marker proteins on the ER or the lamin B receptor–localized membrane in the cytoplasm, suggesting that postmitotic AL assembly occurs independently of the chromatin and ER. Collectively, our results indicate that postmitotic AL assembly is a common cellular event and an intermediate step in NE and NPC assembly and in NE expansion in higher eukaryotic cells.