TLR4/NF-κB signaling pathway gene single nucleotide polymorphisms alter gene expression levels and affect ARDS occurrence and prognosis outcomes

• Published in: Medicine (Baltimore) 2019-06, Vol.98 (26), p.e16029-e16029
• Main Authors: Ding, Yueping, Feng, Qijia, Chen, Jianshi, Song, Jia
• Format: Article
• Language: English
• Subjects: Biomarkers - blood; Clinical Trial/Experimental Study; Female; Gene Expression - genetics; Humans; Interleukin-6 - blood; Interleukin-6 - genetics; Male; Middle Aged; Myeloid Differentiation Factor 88 - blood; Myeloid Differentiation Factor 88 - genetics; NF-kappa B - blood; NF-kappa B - genetics; Polymorphism, Single Nucleotide; Prognosis; Respiratory Distress Syndrome - blood; Respiratory Distress Syndrome - diagnosis; Respiratory Distress Syndrome - genetics; Respiratory Distress Syndrome - mortality; RNA, Messenger - blood; Signal Transduction; Survival Analysis; Toll-Like Receptor 4 - blood; Toll-Like Receptor 4 - genetics; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - genetics
• ISSN: 0025-7974; 1536-5964
• DOI: 10.1097/MD.0000000000016029

To study the occurrence and prognosis of acute respiratory distress syndrome (ARDS) using single nucleotide polymorphisms (SNPs) of TNF-α rs1800629, IL-6 rs1800796, and MyD88 rs7744 loci in the TLR4/NF-κB pathway. Genotypes were analyzed for TNF-α rs1800629, IL-6 rs1800796, and MyD88 rs7744 loci. Plasma TNF-α and IL-6 levels and MyD88 mRNA expression in peripheral blood mononuclear cells (PBMCs) of 300 ARDS patients and 300 non-ARDS patients (control group) were examined. The patients were followed up for 60 days, and the prognosis outcome was recorded. The TNF-α rs1800629 locus A allele and the IL-6 rs1800796 locus G allele were found to be risk factors for ARDS (adjusted OR = 1.452, 95% CI: 1.211-1.689, P