Novel higher-order epigenetic regulation of the Bdnf gene upon seizures

Studies in cultured cells have demonstrated the existence of higher-order epigenetic mechanisms, determining the relationship between expression of the gene and its position within the cell nucleus. It is unknown, whether such mechanisms operate in postmitotic, highly differentiated cell types, such...

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Published in:The Journal of neuroscience 2013-02, Vol.33 (6), p.2507-2511
Main Authors: Walczak, Agnieszka, Szczepankiewicz, Andrzej A, Ruszczycki, Blazej, Magalska, Adriana, Zamlynska, Katarzyna, Dzwonek, Joanna, Wilczek, Ewa, Zybura-Broda, Katarzyna, Rylski, Marcin, Malinowska, Monika, Dabrowski, Michal, Szczepinska, Teresa, Pawlowski, Krzysztof, Pyskaty, Marta, Wlodarczyk, Jakub, Szczerbal, Izabela, Switonski, Marek, Cremer, Marion, Wilczynski, Grzegorz M
Format: Article
Language:English
Subjects:
Animals
Brain-Derived Neurotrophic Factor - genetics
Brain-Derived Neurotrophic Factor - physiology
Brief Communications
Cell Nucleus - genetics
Cell Nucleus - metabolism
Epigenesis, Genetic - physiology
Male
Rats
Rats, Wistar
Receptor, trkB - physiology
Seizures - genetics
Seizures - metabolism
Translocation, Genetic - physiology
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Summary:Studies in cultured cells have demonstrated the existence of higher-order epigenetic mechanisms, determining the relationship between expression of the gene and its position within the cell nucleus. It is unknown, whether such mechanisms operate in postmitotic, highly differentiated cell types, such as neurons in vivo. Accordingly, we examined whether the intranuclear positions of Bdnf and Trkb genes, encoding the major neurotrophin and its receptor respectively, change as a result of neuronal activity, and what functional consequences such movements may have. In a rat model of massive neuronal activation upon kainate-induced seizures we found that elevated neuronal expression of Bdnf is associated with its detachment from the nuclear lamina, and translocation toward the nucleus center. In contrast, the position of stably expressed Trkb remains unchanged after seizures. Our study demonstrates that activation-dependent architectural remodeling of the neuronal cell nucleus in vivo contributes to activity-dependent changes in gene expression in the brain.
ISSN:0270-6474
1529-2401
1529-2401
DOI:10.1523/JNEUROSCI.1085-12.2013