High-Throughput Yeast Aging Analysis for Cryptococcus (HYAAC) microfluidic device streamlines aging studies in Cryptococcus neoformans

• Published in: Communications biology 2019-07, Vol.2 (1), p.256-256, Article 256
• Main Authors: Orner, Erika P., Zhang, Pengchao, Jo, Myeong C., Bhattacharya, Somanon, Qin, Lidong, Fries, Bettina C.
• Format: Article
• Language: English
• Subjects: 13; 13/62; 14/35; 14/63; 38; 38/77; 631/1647/277; 631/326/193/2542; 631/326/22/1434; Age; Aging; Amphotericin B - pharmacology; Anti-Infective Agents - pharmacology; Antifungal Agents - pharmacology; Biology; Biomedical and Life Sciences; Cell death; Cryptococcus; Cryptococcus neoformans; Cryptococcus neoformans - genetics; Cryptococcus neoformans - physiology; Drug Resistance, Microbial; Gene expression; Lab-On-A-Chip Devices; Life Sciences; Life span; Microdissection; Microfluidics; Microfluidics - instrumentation; Pathogens; Phenotype; Phenotypes; RNA - analysis; Saccharomyces cerevisiae; Up-Regulation; Virulence; Yeast
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-019-0504-5

Cryptococcus neoformans (Cn) is a deadly fungal pathogen responsible for ~ 180,000 deaths per year and despite effective antifungals, treatment failure and resistance to antifungals are increasingly problematic. Aging and age-related phenotypes are prominent virulence traits that contribute to the resilience of Cn to host responses and antifungals. Traditional methods to study aging in Cn are expensive, inefficient and in need of improvement. Here, we demonstrate the development and use of a High-Throughput Yeast Aging Analysis for Cryptococcus (HYAAC) microfluidic device to better study aging and age-associated genes in Cn . Compared to traditional methods, the HYAAC is superior in its efficiency to isolate, manipulate and observe old cells for analysis. It allows for the trapping and tracking of individual cells over the course of their lifespan, allowing for more precise measurements of lifespan, tracking of age-related phenotypes with age, and a more high-throughput ability to investigate genes associated with aging. Orner et al. develops a microfluidic device called High-Throughput Yeast Aging Analysis for Cryptococcus (HYAAC) that can be used to measure lifespan, cell death, and dynamics of gene expression changes across age. This device may streamline aging studies in Cryptococcus neoformans and facilitate investigations in yeast pathogens.