Wisteria floribunda agglutinin-positive mac-2 binding protein as an age-independent fibrosis marker in nonalcoholic fatty liver disease

• Published in: Scientific reports 2019-07, Vol.9 (1), p.10109-8, Article 10109
• Main Authors: Tamaki, Nobuharu, Higuchi, Mayu, Kurosaki, Masayuki, Kirino, Sakura, Osawa, Leona, Watakabe, Keiya, Wang, Wan, Okada, Mao, Shimizu, Takao, Takaura, Kenta, Takada, Hitomi, Kaneko, Shun, Yasui, Yutaka, Tsuchiya, Kaoru, Nakanishi, Hiroyuki, Itakura, Jun, Takahashi, Yuka, Enomoto, Nobuyuki, Izumi, Namiki
• Format: Article
• Language: English
• Subjects: 59/57; 692/4020/4021; 692/4020/4021/1607; Age; Age Factors; Aged; Antigens, Neoplasm - blood; Antigens, Neoplasm - metabolism; Biomarkers - blood; Biomarkers - metabolism; Biopsy; Elasticity Imaging Techniques; Fatty liver; Female; Fibrosis; Health risks; Humanities and Social Sciences; Humans; Liver; Liver Cirrhosis - diagnostic imaging; Liver Cirrhosis - metabolism; Liver Cirrhosis - pathology; Liver diseases; Male; Membrane Glycoproteins - blood; Membrane Glycoproteins - metabolism; Middle Aged; Mortality risk; multidisciplinary; Non-alcoholic Fatty Liver Disease - metabolism; Non-alcoholic Fatty Liver Disease - pathology; Plant Lectins - metabolism; Receptors, N-Acetylglucosamine - metabolism; ROC Curve; Science; Science (multidisciplinary); Wisteria floribunda
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-46172-1

The assessment of liver fibrosis is essential because it correlates with mortality risk in nonalcoholic fatty liver disease (NAFLD). This study aims to examine whether serum fibrosis markers could identify candidate patients likely to have advanced fibrosis. We enrolled 352 patients with NAFLD and performed liver biopsies in 97 patients. The area under the receiver operating characteristic curve (AUROC) of liver stiffness by magnetic resonance elastography for histological advanced fibrosis was 0.910, and the optimal cutoff value was 4.07 kPa. To predict severe liver stiffness (≥4.07 kPa), the AUROC for Wisteria floribunda agglutinin-positive mac-2 binding protein (WFA + -M2BP) and FIB-4 were 0.897 (cutoff value, 1.08) and 0.880 (cutoff value, 2.53), respectively. After stratification of patients into four age groups as quartile, the optimal cutoff values of WFA + -M2BP for predicting severe liver stiffness were similar in each group (1.09, 1.08, 1.10, and 1.12). On the other hand, those of FIB-4 increased in parallel with age (1.47, 2.19, 2.99, and 3.88). In conclusion, WFA + -M2BP was precise for estimating severe liver stiffness in NAFLD with single cutoff value independent of age. Hence, identifying high-risk cases using WFA + -M2BP from a large number of NAFLD patients is clinically significant.