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A Wheat β-Patchoulene Synthase Confers Resistance Against Herbivory in Transgenic Arabidopsis

Terpenoids play important roles in plant defense. Although some terpene synthases have been characterized, terpenoids and their biosynthesis in wheat ( L.) still remain largely unknown. Here, we describe the identification of a terpene synthase gene in wheat. It encodes a sesquiterpene synthase that...

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Published in:Genes 2019-06, Vol.10 (6), p.441
Main Authors: Pu, Qingyu, Liang, Jin, Shen, Qinqin, Fu, Jingye, Pu, Zhien, Liu, Jiang, Wang, Xuegui, Wang, Qiang
Format: Article
Language:English
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Summary:Terpenoids play important roles in plant defense. Although some terpene synthases have been characterized, terpenoids and their biosynthesis in wheat ( L.) still remain largely unknown. Here, we describe the identification of a terpene synthase gene in wheat. It encodes a sesquiterpene synthase that catalyzes β-patchoulene formation with -farnesyl diphosphate (FPP) as the substrate, thus named as TaPS. TaPS exhibits inducible expression in wheat in response to various elicitations. Particularly, alamethicin treatment strongly induces gene expression and β-patchoulene accumulation in wheat. Overexpression of in Arabidopsis successfully produces β-patchoulene, verifying the biochemical function of TaPS in planta. Furthermore, these transgenic Arabidopsis plants exhibit resistance against herbivory by repelling beet armyworm larvae feeding, thereby indicating anti-herbivory activity of β-patchoulene. The catalytic mechanism of TaPS is also explored by homology modeling and site-directed mutagenesis. Two key amino acids are identified to act in protonation and stability of intermediates and product formation. Taken together, one wheat sesquiterpene synthase is identified as β-patchoulene synthase. TaPS exhibits inducible gene expression and the sesquiterpene β-patchoulene is involved in repelling insect infestation.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes10060441