Preliminary evaluation of Hitchens-Pike-Todd-Hewitt medium (HPTH) for detection of group B streptococci in pregnant women

We aimed to compare Hitchens–Pike–Todd–Hewitt (HPTH) enrichment broth, Todd–Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplica...

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Published in:Journal of clinical laboratory analysis 2010, Vol.24 (6), p.403-406
Main Authors: Chaves Jr, Maurício, Pádua, Rubia A.F., Campanerut, Paula A.Z., Pelloso, Sandra M., Carvalho, Maria Dalva B., Siqueira, Vera L.D., Scodro, Regiane B.L., Cardoso, Rosilene F.
Format: Article
Language:English
Subjects:
Anal Canal - microbiology
Brazil - epidemiology
Carrier State - diagnosis
Carrier State - epidemiology
Carrier State - microbiology
culture
Culture Media
enrichment broth
Female
group B streptococci
Humans
Mass Screening - methods
neonatal infection
Original
Pregnancy
Pregnancy Complications, Infectious - diagnosis
Pregnancy Complications, Infectious - epidemiology
Pregnancy Complications, Infectious - microbiology
Pregnancy Trimester, Third
pregnant women
Prevalence
Rectum - microbiology
Streptococcal Infections - diagnosis
Streptococcal Infections - epidemiology
Streptococcal Infections - microbiology
Streptococcus - growth & development
Streptococcus - isolation & purification
Vagina - microbiology
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Summary:We aimed to compare Hitchens–Pike–Todd–Hewitt (HPTH) enrichment broth, Todd–Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at Santa Casa de Misericórdia Hospital, Maringá, Paraná, Brazil, were screened by GBS using HPTH, Todd–Hewitt broth, and ABA. Twenty‐five(24.5%) pregnant women had one or more positive culture for GBS in those media. The positive rate for each medium was 21.6% (22/102) in the HPTH, 12.8% (13/102) in ABA, and 11.8% (12/102) in Todd–Hewitt broth. HPTH seems to be a high satisfactory medium for screening GBS in vaginal and anorectal samples in pregnant women and additional studies would be interesting in a larger number of pregnant women and in different laboratories. J. Clin. Lab. Anal. 24:403–406, 2010. © 2010 Wiley‐Liss, Inc.
ISSN:0887-8013
1098-2825
1098-2825
DOI:10.1002/jcla.20419