Immunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane-enriched fractions from infectious Leishmania (Viannia) braziliensis

The diagnosis of American tegumentary leishmaniasis (ATL) is based on the visualization or isolation of the parasite, which is a time‐consuming and poorly sensitive method. In this study, we evaluated the accuracy and reliability of ELISA for the diagnosis of ATL using soluble (SF) and membrane‐enri...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2010, Vol.24 (5), p.289-294
Main Authors: Cataldo, Jamyra Iglesias, de Queiroz Mello, Fernanda Carvalho, Mouta-Confort, Eliame, de Fátima Madeira, Maria, de Oliveira Schubach, Armando, da Silva Genestra, Marcelo, Ribeiro, Flávia Coelho, de Fátima Moreira-Venâncio, Célia, Passos, Sônia Regina Lambert
Format: Article
Language:English
Subjects:
Adult
Antibodies, Protozoan - blood
Antigens, Protozoan - immunology
Brazil
Case-Control Studies
Cell Membrane - immunology
Cytosol - immunology
diagnostic techniques and procedures
ELISA
Enzyme-Linked Immunosorbent Assay
Female
Humans
Leishmania
Leishmania (Viannia) braziliensis
Leishmania braziliensis - immunology
Leishmaniasis, Cutaneous - diagnosis
Leishmaniasis, Cutaneous - immunology
Leishmaniasis, Cutaneous - parasitology
Male
Original
Prognosis
reliability and validity
ROC Curve
Sensitivity and Specificity
validation studies [type of studies]
Viannia braziliensis
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Summary:The diagnosis of American tegumentary leishmaniasis (ATL) is based on the visualization or isolation of the parasite, which is a time‐consuming and poorly sensitive method. In this study, we evaluated the accuracy and reliability of ELISA for the diagnosis of ATL using soluble (SF) and membrane‐enriched (MF) antigen fractions obtained from an infectious strain of Leishmania (Viannia) braziliensis. A total of 152 serum samples investigated at a referral center in Rio de Janeiro, Brazil, between 2005 and 2007 were studied. Each sample was tested twice with each fraction for the calculation of reliability (intraclass coefficient (ICC)). Cut‐off values of 0.22 (SF) and 0.33 (MF) were defined. The use of the fractions resulted in good discrimination between patients, with a large area under the curve (P
ISSN:0887-8013
1098-2825
1098-2825
DOI:10.1002/jcla.20402