Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR

• Published in: Journal of clinical laboratory analysis 2010, Vol.24 (3), p.134-138
• Main Authors: Sener, Kenan, Yapar, Mehmet, Bedir, Orhan, Gül, Cem, Coskun, Ömer, Kubar, Ayhan
• Format: Article
• Language: English
• Subjects: Blood - metabolism; Hepacivirus - genetics; Hepacivirus - isolation & purification; Hepatitis C virus; Humans; Original; Polymerase chain reaction; real-time PCR; Refrigeration; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA Stability; RNA viruses; RNA, Viral - blood; RNA, Viral - metabolism; Specimen Handling - methods; stability of HCV RNA; Storage conditions; TaqMan; Time Factors
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.20354

The storage conditions of blood samples for reliable results are very important in hepatitis C virus (HCV) RNA amplification tests used in routine HCV analyses. According to many studies, storage conditions could affect the RNA stability for HCV RNA detection. We have studied HCV RNA stability in blood samples stored at 4°C. Nineteen blood samples containing different HCV RNA levels were stored at 4°C and they were then analyzed by TaqMAN real‐time PCR method. HCV RNA levels remained almost stable (100%) at least for five weeks at this storage condition. However, among them, the stability period was up to 11 weeks in two of the samples. As with these findings, there was a slightly significant correlation between the positivity time and the beginning HCV RNA levels (r=0.474, P=0.040). We conclude that, blood samples can be stored at 4°C for five weeks without any significant difference in detected HCV RNA level by using TaqMan real‐time PCR. J. Clin. Lab. Anal. 24:134–138, 2010. © 2010 Wiley‐Liss, Inc.