Preliminary clinical measurement of the expression of B-cell activating factor in Chinese systemic lupus erythematosus patients

It has recently been found that excessive serum B‐cell activating factor (BAFF) production triggers severe autoimmune disorders in mice resembling systemic lupus erythematosus (SLE). Whether such dysregulation in circulating levels of BAFF results from overexpression of BAFF gene in clinical patient...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical laboratory analysis 2007, Vol.21 (3), p.183-187
Main Authors: Lin, Zhou, Dan-Rong, Yang, Xiao-Qing, Tu, Hao, Wang, Hong-Lian, Geng, Xian-Tao, Kong, Ren-Qian, Zhong, Jiyu, Li
Format: Article
Language:English
Subjects:
Adult
Antibodies, Antinuclear - blood
Asian Continental Ancestry Group
autoimmune disorders
B lymphocyte
B-Cell Activating Factor - blood
B-Cell Activating Factor - genetics
DNA - immunology
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression
Humans
Immunoglobulins - blood
Leukocytes, Mononuclear - chemistry
Lupus Erythematosus, Systemic - blood
Lupus Erythematosus, Systemic - genetics
Lupus Erythematosus, Systemic - immunology
Male
Middle Aged
Original
reverse transcript-polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
systematic lupus erythematosus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It has recently been found that excessive serum B‐cell activating factor (BAFF) production triggers severe autoimmune disorders in mice resembling systemic lupus erythematosus (SLE). Whether such dysregulation in circulating levels of BAFF results from overexpression of BAFF gene in clinical patients with SLE is still unclear. In this study, we investigated the relationship between the expression of BAFF and SLE. Here, circulating levels of BAFF were measured in 59 Chinese patients with SLE using enzyme‐linked immunosorbent assay (ELISA). In addition, we have quantified the specific mRNA levels of BAFF in unstimulated peripheral‐blood mononuclear cells (PBMNCs) using real‐time polymerase chain reaction (PCR). Serum samples of all SLE patients were also assayed for quantitative immunoglobulins (IgG, IgA, IgM) and anti‐double‐stranded DNA (anti‐dsDNA) antibody levels, and compared with samples from 40 healthy control subjects. Serum BAFF levels in all SLE patients were significantly elevated (P
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.20161