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Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L
Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in is not well understood. Here, we report that the M-locus protein kinase ( ) , the functional homolog of in , controls SI in . We identif...
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| Published in: | International journal of molecular sciences 2019-07, Vol.20 (13), p.3303 |
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| container_title | International journal of molecular sciences |
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| creator | Chen, Fang Yang, Yong Li, Bing Liu, Zhiquan Khan, Fawad Zhang, Tong Zhou, Guilong Tu, Jinxing Shen, Jinxiong Yi, Bin Fu, Tingdong Dai, Cheng Ma, Chaozhi |
| description | Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in
is not well understood. Here, we report that the M-locus protein kinase (
)
, the functional homolog of
in
, controls SI in
. We identified four paralogue
genes in
, including
,
,
, and
. Two transcripts of
,
and
, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that
, especially
, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of
in
SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of
in regulating the SI response of
. We found that the expression of SI positive regulators S-locus receptor kinase (
) and Arm-Repeat Containing 1 (
) are suppressed in
mutant, whereas the self-compatibility (SC) element Glyoxalase I (
) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of
in the SI of |
| doi_str_mv | 10.3390/ijms20133303 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6651594</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2254508151</sourcerecordid><originalsourceid>FETCH-LOGICAL-c478t-a89afd97b1528a398adf30c1cd29efc1546fd3dc6359a659246fc36309a5ac613</originalsourceid><addsrcrecordid>eNpdkc1PFDEYhxuCAURvnk0TLhwcaftOZ6cXEyCixAWNH-fm3U4Huum0SzuzyZ781-0GJKuXfj7v048fIW84ew-g2JlbDlkwDgAM9sgRr4WoGGtm-zvjQ_Iy5yVjAoRUB-QQuGhrUPyI_L6aghldDOjpeWk22WUae3pTzaOZMv2W4mhdoF9cwGzpd7u26G1Hkd7GtfVl4W7yOMa0oTfW3GNwedjW_7C-r66DicMKR7dw3o0bWjwXCXN2BmnAVdHPX5EXPfpsXz_1x-TX1cefl5-r-ddP15fn88rUs3assFXYd2q24FK0CKrFrgdmuOmEsr3hsm76DjrTgFTYSCXK3EADTKFE03A4Jh8evatpMdjO2DAm9HqV3IBpoyM6_e9OcPf6Lq5100guVV0Ep0-CFB8mm0c9uGys9xhsnLIWQtaStVxuzzr5D13GKZXPLVSJSbRSsq3w3SNlUsw52f75MpzpbbJ6N9mCv919wDP8N0r4A3TWoLY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2333285504</pqid></control><display><type>article</type><title>Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Chen, Fang ; Yang, Yong ; Li, Bing ; Liu, Zhiquan ; Khan, Fawad ; Zhang, Tong ; Zhou, Guilong ; Tu, Jinxing ; Shen, Jinxiong ; Yi, Bin ; Fu, Tingdong ; Dai, Cheng ; Ma, Chaozhi</creator><creatorcontrib>Chen, Fang ; Yang, Yong ; Li, Bing ; Liu, Zhiquan ; Khan, Fawad ; Zhang, Tong ; Zhou, Guilong ; Tu, Jinxing ; Shen, Jinxiong ; Yi, Bin ; Fu, Tingdong ; Dai, Cheng ; Ma, Chaozhi</creatorcontrib><description>Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in
is not well understood. Here, we report that the M-locus protein kinase (
)
, the functional homolog of
in
, controls SI in
. We identified four paralogue
genes in
, including
,
,
, and
. Two transcripts of
,
and
, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that
, especially
, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of
in
SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of
in regulating the SI response of
. We found that the expression of SI positive regulators S-locus receptor kinase (
) and Arm-Repeat Containing 1 (
) are suppressed in
mutant, whereas the self-compatibility (SC) element Glyoxalase I (
) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of
in the SI of</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20133303</identifier><identifier>PMID: 31284391</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino Acid Sequence ; Base Sequence ; Brassica napus - enzymology ; Brassica napus - genetics ; Brassica napus - physiology ; Cloning ; CRISPR ; CRISPR-Cas Systems - genetics ; Functional analysis ; Gene Editing ; Gene Expression Regulation, Plant ; Gene frequency ; Genes ; Genomes ; Haplotypes ; Incompatibility ; Kinases ; Ligands ; Mutation ; Mutation - genetics ; Organ Specificity - genetics ; Phylogenetics ; Phylogeny ; Plant reproduction ; Plants, Genetically Modified ; Pollination ; Protein Isoforms - chemistry ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Protein kinase ; Protein Kinase C - chemistry ; Protein Kinase C - genetics ; Protein Kinase C - metabolism ; Proteins ; Rape plants ; RNA Interference ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Roles ; Self-incompatibility ; Self-Incompatibility in Flowering Plants - physiology ; Transcription ; Transgenic plants</subject><ispartof>International journal of molecular sciences, 2019-07, Vol.20 (13), p.3303</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-a89afd97b1528a398adf30c1cd29efc1546fd3dc6359a659246fc36309a5ac613</citedby><cites>FETCH-LOGICAL-c478t-a89afd97b1528a398adf30c1cd29efc1546fd3dc6359a659246fc36309a5ac613</cites><orcidid>0000-0002-4853-8278 ; 0000-0002-4958-7725 ; 0000-0001-5131-4526 ; 0000-0002-0012-2193</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2333285504/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2333285504?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31284391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Fang</creatorcontrib><creatorcontrib>Yang, Yong</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><creatorcontrib>Liu, Zhiquan</creatorcontrib><creatorcontrib>Khan, Fawad</creatorcontrib><creatorcontrib>Zhang, Tong</creatorcontrib><creatorcontrib>Zhou, Guilong</creatorcontrib><creatorcontrib>Tu, Jinxing</creatorcontrib><creatorcontrib>Shen, Jinxiong</creatorcontrib><creatorcontrib>Yi, Bin</creatorcontrib><creatorcontrib>Fu, Tingdong</creatorcontrib><creatorcontrib>Dai, Cheng</creatorcontrib><creatorcontrib>Ma, Chaozhi</creatorcontrib><title>Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in
is not well understood. Here, we report that the M-locus protein kinase (
)
, the functional homolog of
in
, controls SI in
. We identified four paralogue
genes in
, including
,
,
, and
. Two transcripts of
,
and
, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that
, especially
, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of
in
SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of
in regulating the SI response of
. We found that the expression of SI positive regulators S-locus receptor kinase (
) and Arm-Repeat Containing 1 (
) are suppressed in
mutant, whereas the self-compatibility (SC) element Glyoxalase I (
) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of
in the SI of</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Brassica napus - enzymology</subject><subject>Brassica napus - genetics</subject><subject>Brassica napus - physiology</subject><subject>Cloning</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>Functional analysis</subject><subject>Gene Editing</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene frequency</subject><subject>Genes</subject><subject>Genomes</subject><subject>Haplotypes</subject><subject>Incompatibility</subject><subject>Kinases</subject><subject>Ligands</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Organ Specificity - genetics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant reproduction</subject><subject>Plants, Genetically Modified</subject><subject>Pollination</subject><subject>Protein Isoforms - chemistry</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Protein kinase</subject><subject>Protein Kinase C - chemistry</subject><subject>Protein Kinase C - genetics</subject><subject>Protein Kinase C - metabolism</subject><subject>Proteins</subject><subject>Rape plants</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Roles</subject><subject>Self-incompatibility</subject><subject>Self-Incompatibility in Flowering Plants - physiology</subject><subject>Transcription</subject><subject>Transgenic plants</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkc1PFDEYhxuCAURvnk0TLhwcaftOZ6cXEyCixAWNH-fm3U4Huum0SzuzyZ781-0GJKuXfj7v048fIW84ew-g2JlbDlkwDgAM9sgRr4WoGGtm-zvjQ_Iy5yVjAoRUB-QQuGhrUPyI_L6aghldDOjpeWk22WUae3pTzaOZMv2W4mhdoF9cwGzpd7u26G1Hkd7GtfVl4W7yOMa0oTfW3GNwedjW_7C-r66DicMKR7dw3o0bWjwXCXN2BmnAVdHPX5EXPfpsXz_1x-TX1cefl5-r-ddP15fn88rUs3assFXYd2q24FK0CKrFrgdmuOmEsr3hsm76DjrTgFTYSCXK3EADTKFE03A4Jh8evatpMdjO2DAm9HqV3IBpoyM6_e9OcPf6Lq5100guVV0Ep0-CFB8mm0c9uGys9xhsnLIWQtaStVxuzzr5D13GKZXPLVSJSbRSsq3w3SNlUsw52f75MpzpbbJ6N9mCv919wDP8N0r4A3TWoLY</recordid><startdate>20190705</startdate><enddate>20190705</enddate><creator>Chen, Fang</creator><creator>Yang, Yong</creator><creator>Li, Bing</creator><creator>Liu, Zhiquan</creator><creator>Khan, Fawad</creator><creator>Zhang, Tong</creator><creator>Zhou, Guilong</creator><creator>Tu, Jinxing</creator><creator>Shen, Jinxiong</creator><creator>Yi, Bin</creator><creator>Fu, Tingdong</creator><creator>Dai, Cheng</creator><creator>Ma, Chaozhi</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4853-8278</orcidid><orcidid>https://orcid.org/0000-0002-4958-7725</orcidid><orcidid>https://orcid.org/0000-0001-5131-4526</orcidid><orcidid>https://orcid.org/0000-0002-0012-2193</orcidid></search><sort><creationdate>20190705</creationdate><title>Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L</title><author>Chen, Fang ; Yang, Yong ; Li, Bing ; Liu, Zhiquan ; Khan, Fawad ; Zhang, Tong ; Zhou, Guilong ; Tu, Jinxing ; Shen, Jinxiong ; Yi, Bin ; Fu, Tingdong ; Dai, Cheng ; Ma, Chaozhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-a89afd97b1528a398adf30c1cd29efc1546fd3dc6359a659246fc36309a5ac613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Brassica napus - enzymology</topic><topic>Brassica napus - genetics</topic><topic>Brassica napus - physiology</topic><topic>Cloning</topic><topic>CRISPR</topic><topic>CRISPR-Cas Systems - genetics</topic><topic>Functional analysis</topic><topic>Gene Editing</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene frequency</topic><topic>Genes</topic><topic>Genomes</topic><topic>Haplotypes</topic><topic>Incompatibility</topic><topic>Kinases</topic><topic>Ligands</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Organ Specificity - genetics</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant reproduction</topic><topic>Plants, Genetically Modified</topic><topic>Pollination</topic><topic>Protein Isoforms - chemistry</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>Protein kinase</topic><topic>Protein Kinase C - chemistry</topic><topic>Protein Kinase C - genetics</topic><topic>Protein Kinase C - metabolism</topic><topic>Proteins</topic><topic>Rape plants</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Roles</topic><topic>Self-incompatibility</topic><topic>Self-Incompatibility in Flowering Plants - physiology</topic><topic>Transcription</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Fang</creatorcontrib><creatorcontrib>Yang, Yong</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><creatorcontrib>Liu, Zhiquan</creatorcontrib><creatorcontrib>Khan, Fawad</creatorcontrib><creatorcontrib>Zhang, Tong</creatorcontrib><creatorcontrib>Zhou, Guilong</creatorcontrib><creatorcontrib>Tu, Jinxing</creatorcontrib><creatorcontrib>Shen, Jinxiong</creatorcontrib><creatorcontrib>Yi, Bin</creatorcontrib><creatorcontrib>Fu, Tingdong</creatorcontrib><creatorcontrib>Dai, Cheng</creatorcontrib><creatorcontrib>Ma, Chaozhi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Fang</au><au>Yang, Yong</au><au>Li, Bing</au><au>Liu, Zhiquan</au><au>Khan, Fawad</au><au>Zhang, Tong</au><au>Zhou, Guilong</au><au>Tu, Jinxing</au><au>Shen, Jinxiong</au><au>Yi, Bin</au><au>Fu, Tingdong</au><au>Dai, Cheng</au><au>Ma, Chaozhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2019-07-05</date><risdate>2019</risdate><volume>20</volume><issue>13</issue><spage>3303</spage><pages>3303-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in
is not well understood. Here, we report that the M-locus protein kinase (
)
, the functional homolog of
in
, controls SI in
. We identified four paralogue
genes in
, including
,
,
, and
. Two transcripts of
,
and
, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that
, especially
, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of
in
SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of
in regulating the SI response of
. We found that the expression of SI positive regulators S-locus receptor kinase (
) and Arm-Repeat Containing 1 (
) are suppressed in
mutant, whereas the self-compatibility (SC) element Glyoxalase I (
) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of
in the SI of</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31284391</pmid><doi>10.3390/ijms20133303</doi><orcidid>https://orcid.org/0000-0002-4853-8278</orcidid><orcidid>https://orcid.org/0000-0002-4958-7725</orcidid><orcidid>https://orcid.org/0000-0001-5131-4526</orcidid><orcidid>https://orcid.org/0000-0002-0012-2193</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2019-07, Vol.20 (13), p.3303 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6651594 |
| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Amino Acid Sequence Base Sequence Brassica napus - enzymology Brassica napus - genetics Brassica napus - physiology Cloning CRISPR CRISPR-Cas Systems - genetics Functional analysis Gene Editing Gene Expression Regulation, Plant Gene frequency Genes Genomes Haplotypes Incompatibility Kinases Ligands Mutation Mutation - genetics Organ Specificity - genetics Phylogenetics Phylogeny Plant reproduction Plants, Genetically Modified Pollination Protein Isoforms - chemistry Protein Isoforms - genetics Protein Isoforms - metabolism Protein kinase Protein Kinase C - chemistry Protein Kinase C - genetics Protein Kinase C - metabolism Proteins Rape plants RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism Roles Self-incompatibility Self-Incompatibility in Flowering Plants - physiology Transcription Transgenic plants |
| title | Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T21%3A04%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20Analysis%20of%20M-Locus%20Protein%20Kinase%20Revealed%20a%20Novel%20Regulatory%20Mechanism%20of%20Self-Incompatibility%20in%20Brassica%20napus%20L&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Chen,%20Fang&rft.date=2019-07-05&rft.volume=20&rft.issue=13&rft.spage=3303&rft.pages=3303-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms20133303&rft_dat=%3Cproquest_pubme%3E2254508151%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c478t-a89afd97b1528a398adf30c1cd29efc1546fd3dc6359a659246fc36309a5ac613%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2333285504&rft_id=info:pmid/31284391&rfr_iscdi=true |