Screening the Best Compatibility of Selaginella moellendorffii Prescription on Hyperuricemia and Gouty Arthritis and Its Mechanism

Objectives. The Selaginella moellendorffii prescription (SMP) consists of S. moellendorffii Herba (SM), Smilacis glabrae Rhizoma (SGR), and Plantaginis Semen (PS). It has been commonly used to treat hyperuricemia and acute gouty arthritis as a hospital preparation. This study was aimed at investigat...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2019, Vol.2019 (2019), p.1-10
Main Authors: Chen, Keli, Zhao, Ping, Cheng, Jing, Zhang, Xue-yan, Li, Juan
Format: Article
Language:English
Subjects:
Animal research
Anti-inflammatory agents
Arthritis
Cell culture
Chemical compounds
Chinese medicine
Chromatography
Creatinine
Edema
Gene expression
Gout
Herbal medicine
High-performance liquid chromatography
Hyperuricemia
IL-1β
Immunofluorescence
Inflammation
Interleukin 8
Lipopolysaccharides
Liquid chromatography
Liver
Metabolism
Mice
NF-κB protein
Nitric oxide
Nuclear transport
Penicillin
Pharmaceuticals
Potassium
Protein transport
Rheumatism
Selaginella moellendorffii
Toxicity
Translocation
Urea
Uric acid
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Summary:Objectives. The Selaginella moellendorffii prescription (SMP) consists of S. moellendorffii Herba (SM), Smilacis glabrae Rhizoma (SGR), and Plantaginis Semen (PS). It has been commonly used to treat hyperuricemia and acute gouty arthritis as a hospital preparation. This study was aimed at investigating the best compatibility ratio of SMP on hyperuricemia and gouty arthritis and getting better insight of the possible mechanism. Methods. In vitro, anti-inflammatory activity of SMP was evaluated by lipopolysaccharide (LPS) induced RAW264.7 cells. The release of nitric oxide (NO) was screened by Griess assay, and NF-κB p65 and NLRP3 proteins expression was examined by immunofluorescence assay. Then, the levels of creatinine (Cr), blood urea nitrogen (BUN), and uric acid (UA) were detected in mice induced by potassium oxonate, and the paw oedema, inflammatory mediators, and histological examination were analyzed in rats induced by monosodium urate (MSU). HPLC method was employed to investigate the chemical profile of this preparation. Results. In vitro, SMP-3 (the ratio of SMP:SGR:PS was 3:1:1) exhibited the most potent anti-NO production activity without obvious toxicity. This anti-inflammatory effect was associated with suppression of NF-κB p65 nuclear translocation and NLRP3 protein expression. In animal experiments, the levels of BUN and Cr in SMP-3 group were lower than other extract groups, and the level of UA was also remarkably decreased by SMP-3 in hyperuricemic mice (P
ISSN:1741-427X
1741-4288
DOI:10.1155/2019/7263034