Grapefruit juice exerts anti-osteoporotic activities in a prednisolone-induced osteoporosis rat femoral fracture model, possibly via the RANKL/OPG axis

This study aimed to shed light on the protective and therapeutic anti-osteoporotic effects and mechanisms of action of grapefruit juice (GFJ) on prednisolone-induced osteoporosis a rat femoral fracture model. We found that treating rats with GFJ before and/or after prednisolone-induced osteoporosis...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2019-08, Vol.71 (4), p.769-783
Main Authors: Bastawy, Eslam Muhammad, Ahmed, Rasha Rashad, Abd El-Hafeez, Amer Ali, Abd El-Hady, Fatma Kamal, Hosoi, Toru, Ozawa, Koichiro, El-Ganzuri, Monir Ali
Format: Article
Language:English
Subjects:
Acid phosphatase (tartrate-resistant)
Alkaline phosphatase
Animals
Biochemistry
Biomedicine
Biotechnology
Bone density
Bone mineral content
Calcium
Chemistry
Chemistry and Materials Science
Drug development
Femur
Flavonoids
Fractures
Immunohistochemistry
Insulin
Original
Original Article
Osteocalcin
Osteoporosis
Osteoprotegerin
Phosphatase
Polyphenols
Prednisolone
TRANCE protein
Vitamin C
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Summary:This study aimed to shed light on the protective and therapeutic anti-osteoporotic effects and mechanisms of action of grapefruit juice (GFJ) on prednisolone-induced osteoporosis a rat femoral fracture model. We found that treating rats with GFJ before and/or after prednisolone-induced osteoporosis resulted in increased bone density, total mineral content, and calcium content to counteract the osteoporotic effects of prednisolone. In parallel, the histological and ultrastructural results of the GFJ-treated groups correlated well with enhanced breaking strength of femurs subjected to a constant load. Furthermore, GFJ treatment before and after prednisolone-induced osteoporosis decreased plasma alkaline phosphatase and tartrate-resistant acid phosphatase activities and increased the level of insulin-like growth factor 1. Mechanistically, our immunohistochemistry study showed that GFJ ameliorated prednisolone-induced osteocalcin depletion, decreased receptor activator of nuclear factor kappa-B ligand (RANKL) expression, and increased osteoprotegerin (OPG) expression. GFJ showed a beneficial anti-osteoporotic effect against prednisolone-induced osteoporosis in rats, possibly via the RANKL/OPG axis, suggesting that GFJ might be a good candidate for developing anti-osteoporotic drugs.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-019-00321-6