In vitro therapeutic effect of Hemiscorpius lepturus venom on tachyzoites of Toxoplasma gondii

Pyrimethamine which is a main anti- Toxoplasma gondii drug has a serious side and toxic effects on the host. Accordingly, the development of new treatment options for toxoplasmosis with less toxic effects, low teratogenicity and parasiticidal effect against the various stage of T. gondii are dramati...

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Bibliographic Details
Published in:Journal of parasitic diseases 2019-09, Vol.43 (3), p.472-478
Main Authors: Khaleghi Rostamkolaie, L., Hamidinejat, H., Razi Jalali, M. H., Jafari, H., Najafzadeh Varzi, H., Seifi Abadshapouri, M. R.
Format: Article
Language:English
Subjects:
Colorimetry
Cytotoxicity
Habromys lepturus
Health Promotion and Disease Prevention
Infectious Diseases
Medicine
Medicine & Public Health
Mitochondria
Original
Original Article
Oxidation
Protozoa
Pyrimethamine
Tachyzoites
Teratogenicity
Toxoplasma gondii
Toxoplasmosis
Venom
Vero cells
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Summary:Pyrimethamine which is a main anti- Toxoplasma gondii drug has a serious side and toxic effects on the host. Accordingly, the development of new treatment options for toxoplasmosis with less toxic effects, low teratogenicity and parasiticidal effect against the various stage of T. gondii are dramatically crucial. Currently, natural molecules from scorpion and snake venoms are widely used as an alternative treatment against human disease, these compounds considered to be safe and to have low toxicity in comparison with synthetic drugs. Therefore, the goal of our study was to investigate the anti- Toxoplasma gondii activities of Hemiscorpius lepturus venom. We measured cytotoxicity of H. lepturus whole venom on Vero cells as well as effectiveness of this compound on viability of T. gondii applying colorimetric assay, according to mitochondrial oxidation of the MTT reagent (Methylthiazol tetrazolium 98%). The results of this study indicated that the H. lepturus whole venom has an anti- Toxoplasma effects with less toxic effect on Vero cells. Also, the T. gondii tachyzoites were treated with H. lepturus venom reached better results in comparison with Pyrimethamine-treated group. This research will serve as a base for future studies on toxoplasmosis and suggest a role for scorpion venom in promoting natural drugs.
ISSN:0971-7196
0975-0703
DOI:10.1007/s12639-019-01113-7