RAB38 is a potential prognostic factor for tumor recurrence in non-small cell lung cancer

Ras-related protein Rab-38 (RAB38) is a member of the Ras small G protein family that regulates intracellular vesicular trafficking. Although the expression of is reportedly deregulated in several types of cancer, its role in tumor biology remains to be elucidated. In the present study, the expressi...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2019-09, Vol.18 (3), p.2598-2604
Main Authors: Hsieh, Jia-Juan, Hou, Ming-Mo, Chang, John Wen-Cheng, Shen, Yung-Chi, Cheng, Hsin-Yi, Hsu, Todd
Format: Article
Language:English
Subjects:
Analysis
Cancer metastasis
Development and progression
EDTA
Epidermal growth factors
G proteins
Growth factors
Histology
Lung cancer
Medical prognosis
Metastasis
Non-small cell lung cancer
Patients
Polymerase chain reaction
Prognosis
Proteins
Recurrence (Disease)
RNA
Small cell lung cancer
Surgery
Transcription (Genetics)
Tumors
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Summary:Ras-related protein Rab-38 (RAB38) is a member of the Ras small G protein family that regulates intracellular vesicular trafficking. Although the expression of is reportedly deregulated in several types of cancer, its role in tumor biology remains to be elucidated. In the present study, the expression of was analyzed in tumor specimens from patients with non-small cell lung cancer (NSCLC) with tumor recurrence within 4 years (Group R), and those remaining disease-free following initial surgery (Group NR), by reverse transcription-semi-quantitative PCR and subsequent semi-quantification using ImageJ v4.0 software. The results revealed that the expression of in Group R and NR specimens was positively associated with tumor recurrence; a high expression level was also associated with poor survival rate in these patients. Using NSCLC cell lines, it was demonstrated that tumor cells with mutations in the active epidermal growth factor receptor (EGFR) gene expressed higher levels of RAB38 compared with those with the wild-type gene by reverse transcription-PCR and western blot analysis. Furthermore, following specific RAB38 gene knockdown by short hairpin RNA transfection, mutants exhibited markedly reduced invasiveness when compared with cells transfected with empty vector controls by Matrigel Transwell assays. These results suggest that is an important prognostic factor in NSCLC, and may serve a critical role in NSCLC-associated tumor metastasis.
ISSN:1792-1074
1792-0981
1792-1082
DOI:10.3892/ol.2019.10547