Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique

Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-07, Vol.91 (14), p.9018-9024
Main Authors: Pan, Ning, Standke, Shawna J, Kothapalli, Naga Rama, Sun, Mei, Bensen, Ryan C, Burgett, Anthony W. G, Yang, Zhibo
Format: Article
Language:English
Subjects:
Analytical chemistry
Antineoplastic Agents - analysis
Cell Line, Tumor
Chemistry
Heterogeneity
Humans
Ions
Irinotecan
Irinotecan - analysis
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
Population studies
Scientific imaging
Single-Cell Analysis - methods
Spectroscopy
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Summary:Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using the Single-probe MS technique. The method was successfully used to perform rapid absolute quantifications of the anticancer drug irinotecan in individual mammalian cancer cells under ambient conditions in real time. Traditional liquid chromatography/mass spectrometry (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Single-probe qSCMS. This technique showcases heterogeneity of drug efficacy on the single-cell level.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.9b01311