Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. Th...

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Bibliographic Details
Published in:Materials 2019-07, Vol.12 (14), p.2311
Main Authors: Edrees, Hadeel Y, Abu Zeid, Sawsan T H, Atta, Hazem M, AlQriqri, Mehal A
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Antibiotics
Bioceramics
Biomedical materials
Cell culture
Cell growth
Cytotoxicity
Differentiation (biology)
Gene expression
Phosphatase
Polymerase chain reaction
Proteins
Repair
Stem cells
Values
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Summary:This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma12142311