Extra Virgin Olive Oil Contains a Phenolic Inhibitor of the Histone Demethylase LSD1/KDM1A

• Published in: Nutrients 2019-07, Vol.11 (7), p.1656
• Main Authors: Cuyàs, Elisabet, Gumuzio, Juan, Lozano-Sánchez, Jesús, Carreras, David, Verdura, Sara, Llorach-Parés, Laura, Sanchez-Martinez, Melchor, Selga, Elisabet, Pérez, Guillermo J, Scornik, Fabiana S, Brugada, Ramon, Bosch-Barrera, Joaquim, Segura-Carretero, Antonio, Martin, Ángel G, Encinar, José Antonio, Menendez, Javier A
• Format: Article
• Language: English
• Subjects: adenosine; Aldehydes - analysis; Aldehydes - pharmacology; Binding; binding sites; Binding Sites - drug effects; Breast cancer; Breast Neoplasms; Cell Line, Tumor; Chromatin; Co-Repressor Proteins - drug effects; Communication; Computer applications; computer simulation; Disruption; Embryo cells; Enzymatic activity; Enzymes; Epigenetics; extra-virgin olive oil; Gene expression; Gene Expression - drug effects; histone demethylases; Histone Demethylases - antagonists & inhibitors; Histone Demethylases - chemistry; Histone Demethylases - metabolism; Histone H3; Histones; Histones - metabolism; Humans; in vitro studies; lysine; MCF-7 Cells; Metabolic disorders; Models, Molecular; Molecular Docking Simulation; Molecular Structure; neoplasms; Neoplastic Stem Cells - metabolism; nervous system diseases; Oils & fats; Olive oil; Olive Oil - chemistry; Oncology; Peptides; Phenolic compounds; Phenols; Phenols - analysis; Phenols - pharmacology; Pluripotency; Polyphenols; promoter regions; Proteins; Recombinant Proteins - drug effects; Scaffolding; Seminoma; Software; Somatic cells; SOXB1 Transcription Factors - antagonists & inhibitors; SOXB1 Transcription Factors - genetics; Stem cells; Teratocarcinoma; Transcription activation; transcription factors; Tumor cells; Tumorigenesis; Tumors
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11071656

The lysine-specific histone demethylase 1A (LSD1) also known as lysine (K)-specific demethylase 1A (KDM1A) is a central epigenetic regulator of metabolic reprogramming in obesity-associated diseases, neurological disorders, and cancer. Here, we evaluated the ability of oleacein, a biophenol secoiridoid naturally present in extra virgin olive oil (EVOO), to target LSD1. Molecular docking and dynamic simulation approaches revealed that oleacein could target the binding site of the LSD1 cofactor flavin adenosine dinucleotide with high affinity and at low concentrations. At higher concentrations, oleacein was predicted to target the interaction of LSD1 with histone H3 and the LSD1 co-repressor (RCOR1/CoREST), likely disturbing the anchorage of LSD1 to chromatin. AlphaScreen-based in vitro assays confirmed the ability of oleacein to act as a direct inhibitor of recombinant LSD1, with an IC as low as 2.5 μmol/L. Further, oleacein fully suppressed the expression of the transcription factor SOX2 (SEX determining Region Y-box 2) in cancer stem-like and induced pluripotent stem (iPS) cells, which specifically occurs under the control of an LSD1-targeted distal enhancer. Conversely, oleacein failed to modify ectopic SOX2 overexpression driven by a constitutive promoter. Overall, our findings provide the first evidence that EVOO contains a naturally occurring phenolic inhibitor of LSD1, and support the use of oleacein as a template to design new secoiridoid-based LSD1 inhibitors.