Podoplanin and PROX1 Expression in Hypercaloric Diet-induced Pancreatic Injuries

The role of podoplanin (PDPN) and homebox prospero gene 1 (PROX1) in early stages of pancreatic islet changes induced by hypercaloric diet is unclear. The aim of this study was to study PDPN and PROX1 variability in pancreatic islets after a hypercaloric diet in a rat experimental model. Pancreatic...

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Bibliographic Details
Published in:In vivo (Athens) 2019-07, Vol.33 (4), p.1157-1163
Main Authors: Taran, Delia, Tarlui, Valeria Nicoleta, Ceausu, Raluca Amalia, Cimpean, Anca Maria, Raica, Marius, Sarb, Simona
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Biopsy
Diet - adverse effects
Energy Intake
Gene Expression
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Immunohistochemistry
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Pancreatic Diseases - diagnosis
Pancreatic Diseases - etiology
Pancreatic Diseases - pathology
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tumor Suppressor Proteins - genetics
Tumor Suppressor Proteins - metabolism
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Summary:The role of podoplanin (PDPN) and homebox prospero gene 1 (PROX1) in early stages of pancreatic islet changes induced by hypercaloric diet is unclear. The aim of this study was to study PDPN and PROX1 variability in pancreatic islets after a hypercaloric diet in a rat experimental model. Pancreatic biopsies harvested from Sprague-Dawley rats at 3, 6, and 9 weeks following hypercaloric diet intake were evaluated for morphological and molecular changes of Langerhans islets based on PDPN and PROX1 expression Results: Six weeks of hypercaloric diet induced hypertrophy of pancreatic islets with focal expression of Pdpn and Prox1 mRNA. At 9 weeks of hypercaloric diet, strong peri-insular inflammation was found around hypertrophic islets highly expressing PDPN, and lacking Prox1 mRNA and protein expression. This is the first report of Pdpn and Prox1 mRNA expression variability and involvement in early steps of pancreatic islet changes following hypercaloric food intake.
ISSN:0258-851X
1791-7549
DOI:10.21873/invivo.11586