Association of Cyclin Dependent Kinase 10 and Transcription Factor 2 during Human Corneal Epithelial Wound Healing in vitro model

Proper wound healing is dynamic in order to maintain the corneal integrity and transparency. Impaired or delayed corneal epithelial wound healing is one of the most frequently observed ocular defect and difficult to treat. Cyclin dependen kinase (cdk), a known cell cycle regulator, required for prop...

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Bibliographic Details
Published in:Scientific reports 2019-08, Vol.9 (1), p.11802-11802, Article 11802
Main Authors: Zehra, Meraj, Mushtaq, Shamim, Ghulam Musharraf, Syed, Ghani, Rubina, Ahmed, Nikhat
Format: Article
Language:English
Subjects:
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Cell adhesion & migration
Cell cycle
Cell migration
Cornea
Cyclin-dependent kinase
Epithelial cells
Ets-2 protein
Gene expression
Humanities and Social Sciences
Medical treatment
multidisciplinary
Science
Science (multidisciplinary)
Wound healing
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Summary:Proper wound healing is dynamic in order to maintain the corneal integrity and transparency. Impaired or delayed corneal epithelial wound healing is one of the most frequently observed ocular defect and difficult to treat. Cyclin dependen kinase (cdk), a known cell cycle regulator, required for proper proliferating and migration of cell. We therefore investigated the role of cell cycle regulator cdk10, member of cdk family and its functional association with transcriptional factor (ETS2) at active phase of corneal epithelial cell migration. Our data showed that cdk10 was associated with ETS2, while its expression was upregulated at the active phase (18 hours) of cell migration and gradually decrease as the wound was completely closed. Topical treatment with anti-cdk10 and ETS2 antibodies delayed the wound closure time at higest concentration (10 µg/ml) compared to control. Further, our results also showed increased mRNA expression of cdk10 and ETS2 at active phase of migration at approximately 2 fold. Collectively, our data reveals that cdk10 and ETS2 efficiently involved during corneal wound healing. Further studies are warranted to better understand the mechanism and safety of topical cdk10 and ETS2 proteins in corneal epithelial wound-healing and its potential role for human disease treatment.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-48092-6