Danzhi Jiangtang Capsule Mediates NIT-1 Insulinoma Cell Proliferation and Apoptosis by GLP-1/Akt Signaling Pathway

Objective. This study aimed to investigate the effects of Danzhi Jiangtang Capsule (DJC) on the proliferation and apoptosis functions of NIT-1 pancreatic β-cells exposed to high-glucose load through GLP-1 activated Akt/ FoxO1 signaling pathway. Methods. Cellular apoptosis of NIT-1 pancreatic β-cells...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2019, Vol.2019 (2019), p.1-7
Main Authors: Lv, Ming-An, Wang, Yu-Feng, Chen, Mei-Qiao, Fang, Zhao-Hui, Wu, Yuan-Bo, Wu, Yuan-Jie, Wu, Chuan-Yun
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
Annexin V
Apoptosis
Bcl-2 protein
Beta cells
Caspase
Caspase-3
Cell proliferation
Cholecystokinin
Dextrose
Diabetes
Diabetes mellitus
Diabetes mellitus (non-insulin dependent)
Drug dosages
Ethylenediaminetetraacetic acid
Forkhead protein
FOXO1 protein
Gene expression
Glucose
Homeobox
Hyperglycemia
Inhibition
Insulin
Insulin resistance
Insulinoma
Kinases
Metabolism
Neuroendocrine tumors
Oxidative stress
Pancreas
Pancreatic beta cells
Peptides
Proteins
RNA
Signal transduction
Toxicity
Transcription factors
Type 2 diabetes
Western blotting
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Summary:Objective. This study aimed to investigate the effects of Danzhi Jiangtang Capsule (DJC) on the proliferation and apoptosis functions of NIT-1 pancreatic β-cells exposed to high-glucose load through GLP-1 activated Akt/ FoxO1 signaling pathway. Methods. Cellular apoptosis of NIT-1 pancreatic β-cells was induced by culturing in medium with 33.3mmol/L high glucose (HG). Then low-dose DJC (HG +LD), high-dose DJC (HG +HD), high-dose DJC+ GLP-1 inhibition (HG +HD +GI), and high-dose DJC+AKT inhibition (HG +HD+AI) were added, respectively. Cellular proliferation was accessed by cell counting kit (CCK-8) and cellular apoptosis was measured by Annexin V-FITC/PI staining. The protein levels of phosphorylated phosphatidylinositol-3-kinase (p-PI3K), phosphorylated AKT (p-AKT), phosphorylated Forkhead box protein O1 (p-FoxO1), and cleaved caspase-3 were detected by Western blotting. The mRNA expression of pancreatic duodenal homeobox-1 (PDX-1), CyclinD1, Bcl-2, and insulin was tested by Q-PCR. Results. Comparing to HG group, (HG+HD) group showed a significantly increased cellular proliferation. The apoptosis of NIT-1 cells also was obviously reduced, with downregulated cleaved caspase-3 protein level and upregulated PDX-1, CyclinD1, and Bcl-2 mRNA levels (P
ISSN:1741-427X
1741-4288
DOI:10.1155/2019/5356825