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Bta-miR-124a Affects Lipid Metabolism by Regulating PECR Gene

According to our previous studies, bta-miR-124a was differentially expressed in breast tissue between high-fat and low-fat dairy cows. However, the function of bta-miR-124a in lipid metabolism of dairy cows and the identification of its target genes have not been reported. Therefore, this study will...

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Published in:BioMed research international 2019, Vol.2019 (2019), p.1-9
Main Authors: Zhao, Zhihui, Wu, Rui, Liu, Shengjun, Dong, Wentao, Nie, Lian, Liu, Juan, Zou, Ziwen, Han, Shuo, Yang, Zhuonina, Shen, Binglei, Li, E.
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cited_by cdi_FETCH-LOGICAL-c499t-9eea5d75888a2b3e8af902f4f657692e44eddb4c8087b28f99aed24d5ab8608d3
cites cdi_FETCH-LOGICAL-c499t-9eea5d75888a2b3e8af902f4f657692e44eddb4c8087b28f99aed24d5ab8608d3
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container_issue 2019
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container_title BioMed research international
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creator Zhao, Zhihui
Wu, Rui
Liu, Shengjun
Dong, Wentao
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Liu, Juan
Zou, Ziwen
Han, Shuo
Yang, Zhuonina
Shen, Binglei
Li, E.
description According to our previous studies, bta-miR-124a was differentially expressed in breast tissue between high-fat and low-fat dairy cows. However, the function of bta-miR-124a in lipid metabolism of dairy cows and the identification of its target genes have not been reported. Therefore, this study will identify the target gene of bta-miR-124a and explore its role in the regulation of milk lipid metabolism. First, preliminary bioinformatics prediction of bta-miR-124a candidate target genes was performed, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze relative expression changes of bta-miR-124a and its candidate target genes and the expression level of the downstream gene of the target gene in the lipid metabolism signaling pathway in dairy mammary epithelial cell lines (Mac-T), using the dual luciferase reporter system for the identification of the targeting relationship between bta-miR-124a and the candidate target gene. Then, the effect of transfection of bta-miR-124a mimics and inhibitors on triglyceride (TG) and free fatty acid (FFA) levels was analyzed. The results indicate that bta-miR-124a directly interacts with the 3′-untranslated region of peroxisomal trans-2-enoyl-CoA reductase (PECR) to downregulate its expression in Mac-T cells. Further, bta-mir-124a regulates the expression of PECR and the downstream gene extension of very long chain fatty acid protein 2 (ELOVL2) through an unsaturated fatty acid biosynthesis signaling pathway. In conclusion, bta-miR-124a is involved in lipid metabolism by directly downregulating the PECR gene and affecting the expression of the downstream gene ELOVL2 and regulates the content of some key secretory elements such as TG and FFA. The function of bta-miR-124a has a certain effect on the synthesis and secretion of milk fat in the mammary epithelial cells of dairy cows.
doi_str_mv 10.1155/2019/2596914
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However, the function of bta-miR-124a in lipid metabolism of dairy cows and the identification of its target genes have not been reported. Therefore, this study will identify the target gene of bta-miR-124a and explore its role in the regulation of milk lipid metabolism. First, preliminary bioinformatics prediction of bta-miR-124a candidate target genes was performed, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze relative expression changes of bta-miR-124a and its candidate target genes and the expression level of the downstream gene of the target gene in the lipid metabolism signaling pathway in dairy mammary epithelial cell lines (Mac-T), using the dual luciferase reporter system for the identification of the targeting relationship between bta-miR-124a and the candidate target gene. Then, the effect of transfection of bta-miR-124a mimics and inhibitors on triglyceride (TG) and free fatty acid (FFA) levels was analyzed. The results indicate that bta-miR-124a directly interacts with the 3′-untranslated region of peroxisomal trans-2-enoyl-CoA reductase (PECR) to downregulate its expression in Mac-T cells. Further, bta-mir-124a regulates the expression of PECR and the downstream gene extension of very long chain fatty acid protein 2 (ELOVL2) through an unsaturated fatty acid biosynthesis signaling pathway. In conclusion, bta-miR-124a is involved in lipid metabolism by directly downregulating the PECR gene and affecting the expression of the downstream gene ELOVL2 and regulates the content of some key secretory elements such as TG and FFA. 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However, the function of bta-miR-124a in lipid metabolism of dairy cows and the identification of its target genes have not been reported. Therefore, this study will identify the target gene of bta-miR-124a and explore its role in the regulation of milk lipid metabolism. First, preliminary bioinformatics prediction of bta-miR-124a candidate target genes was performed, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze relative expression changes of bta-miR-124a and its candidate target genes and the expression level of the downstream gene of the target gene in the lipid metabolism signaling pathway in dairy mammary epithelial cell lines (Mac-T), using the dual luciferase reporter system for the identification of the targeting relationship between bta-miR-124a and the candidate target gene. Then, the effect of transfection of bta-miR-124a mimics and inhibitors on triglyceride (TG) and free fatty acid (FFA) levels was analyzed. The results indicate that bta-miR-124a directly interacts with the 3′-untranslated region of peroxisomal trans-2-enoyl-CoA reductase (PECR) to downregulate its expression in Mac-T cells. Further, bta-mir-124a regulates the expression of PECR and the downstream gene extension of very long chain fatty acid protein 2 (ELOVL2) through an unsaturated fatty acid biosynthesis signaling pathway. In conclusion, bta-miR-124a is involved in lipid metabolism by directly downregulating the PECR gene and affecting the expression of the downstream gene ELOVL2 and regulates the content of some key secretory elements such as TG and FFA. The function of bta-miR-124a has a certain effect on the synthesis and secretion of milk fat in the mammary epithelial cells of dairy cows.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>31467878</pmid><doi>10.1155/2019/2596914</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9170-0517</orcidid><orcidid>https://orcid.org/0000-0002-6232-580X</orcidid><orcidid>https://orcid.org/0000-0002-4438-6246</orcidid><oa>free_for_read</oa></addata></record>
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subjects Animals
Binding sites
Bioinformatics
Biology
Biosynthesis
Brain cancer
Brain research
Breast
Breast cancer
Cattle
Cell Line
Cell lines
Dairy cattle
Dairy products
Dehydrogenases
Epithelial cells
Fatty Acid Elongases - genetics
Fatty acids
Fatty Acids, Nonesterified - genetics
Fatty Acids, Nonesterified - metabolism
Female
Gene expression
Gene Expression Regulation - genetics
Genes
Humans
Kinases
Lipid metabolism
Lipid Metabolism - genetics
Lipids
Lymphocytes
Lymphocytes T
Mammary gland
Mammary Glands, Animal - cytology
Mammary Glands, Animal - metabolism
Metabolism
MicroRNAs
MicroRNAs - genetics
Milk
Milk - metabolism
Oils & fats
Oxidoreductases Acting on CH-CH Group Donors
Polymerase chain reaction
Proteins
Reductases
Signal transduction
Signaling
Studies
T cells
Target recognition
trans-2-Enoyl-CoA reductase
Transfection
Triglycerides
Triglycerides - genetics
Triglycerides - metabolism
title Bta-miR-124a Affects Lipid Metabolism by Regulating PECR Gene
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