A rat model of serous borderline ovarian tumors induced by 7,12-dimethylbenz[a]anthracene

Serous borderline ovarian tumors (SBOTs) behave between benign cystadenomas and carcinomas, and the effective detection and clinical management of SBOTs remain clinical challenges. Because it is difficult to isolate and enrich borderline tumor cells, a borderline animal model is in need. 7,12-dimeth...

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Bibliographic Details
Published in:Experimental Animals 2019, Vol.68(3), pp.257-265
Main Authors: Cai, Song-Qi, Li, Ying, Li, Yong-Ai, Wang, Li, Zhu, Jian, Zhao, Shu-Hui, Li, Xin, Qiang, Jin-Wei
Format: Article
Language:English
Subjects:
9,10-Dimethyl-1,2-benzanthracene
animal model
Animal models
Anthracene
Benign
Biomarkers
Cyclin D1
dimethylbenz[a]anthracene
Immunoreactivity
Magnetic resonance imaging
Morphology
Original
Ovarian carcinoma
ovarian tumor
p53 Protein
Pathogenesis
serous borderline tumor
Sprague-Dawley rat
Tumor cells
Tumors
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Summary:Serous borderline ovarian tumors (SBOTs) behave between benign cystadenomas and carcinomas, and the effective detection and clinical management of SBOTs remain clinical challenges. Because it is difficult to isolate and enrich borderline tumor cells, a borderline animal model is in need. 7,12-dimethylbenz[a]anthracene (DMBA) is capable of inducing the initiation, promotion, and progression of serous ovarian tumors. This study aims to investigate the proper dosage and induction time of DMBA for rat models of SBOTs, and explore their morphological features demonstrated by magnetic resonance (MR) imaging and molecular genetic characteristics. Rats were randomly divided into six groups (1 mg/70 D, 2 mg/70 D, 3 mg/70 D, 2 mg/50 D, 2 mg/90 D, and 2 mg/110 D). The 3 mg/70 D group induced the most SBOTs (50.0%, 12/24). The micropapillary projections were shown on MR imaging, which was the characteristic of SBOTs. The Cyclin D1 characterizing an early pathogenetic event strongly expressed in induced serous benign tumors (SBTs). The immunoreactivity staining scores of P53 expression significantly increased from SBTs, SBOTs to serous ovarian carcinomas (SCAs), which elucidate that P53 might be a promising biomarker to grade serous ovarian tumors. Based on morphological and molecular genetic similarities, this rodent SBOT model was suitable for investigating the pathogenesis of serous ovarian tumors and developing an early detection strategy.
ISSN:1341-1357
1881-7122
DOI:10.1538/expanim.18-0103