Systematic identification and characterization of Aedes aegypti long noncoding RNAs (lncRNAs)

Long noncoding RNAs (lncRNAs) play diverse roles in biological processes. Aedes aegypti ( Ae . aegypti ), a blood-sucking mosquito, is the principal vector responsible for replication and transmission of arboviruses including dengue, Zika, and Chikungunya virus. Systematic identification and develop...

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Bibliographic Details
Published in:Scientific reports 2019-08, Vol.9 (1), p.12147-9, Article 12147
Main Authors: Azlan, Azali, Obeidat, Sattam M., Yunus, Muhammad Amir, Azzam, Ghows
Format: Article
Language:English
Subjects:
3' Untranslated Regions
45/91
5' Untranslated Regions
631/114/2114
631/208/212/2019
631/337/384/2568
Aedes - genetics
Aedes - growth & development
Aedes aegypti
Animals
Annotations
Antisense RNA
Aquatic insects
Cluster Analysis
Dengue fever
Disease transmission
Embryos
Gene expression
Gene Expression Regulation, Developmental
Genome
Genomes
Humanities and Social Sciences
Identification
Maternal inheritance
Mosquitoes
multidisciplinary
Open Reading Frames
Organisms
Pipelines
Proteins
RNA, Antisense - genetics
RNA, Antisense - metabolism
RNA, Long Noncoding - metabolism
RNA, Messenger - metabolism
RNA-Seq
Science
Science (multidisciplinary)
Vector-borne diseases
Zika virus
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Summary:Long noncoding RNAs (lncRNAs) play diverse roles in biological processes. Aedes aegypti ( Ae . aegypti ), a blood-sucking mosquito, is the principal vector responsible for replication and transmission of arboviruses including dengue, Zika, and Chikungunya virus. Systematic identification and developmental characterisation of Ae . aegypti lncRNAs are still limited. We performed genome-wide identification of lncRNAs, followed by developmental profiling of lncRNA in Ae . aegypti . We identified a total of 4,689 novel lncRNA transcripts, of which 2,064, 2,076, and 549 were intergenic, intronic, and antisense respectively. Ae . aegypti lncRNAs share many characteristics with other species including low expression, low GC content, short in length, and low conservation. Besides, the expression of Ae . aegypti lncRNAs tend to be correlated with neighbouring and antisense protein-coding genes. A subset of lncRNAs shows evidence of maternal inheritance; hence, suggesting potential role of lncRNAs in early-stage embryos. Additionally, lncRNAs show higher tendency to be expressed in developmental and temporal specific manner. The results from this study provide foundation for future investigation on the function of Ae . aegypti lncRNAs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-47506-9