High possibility of hepatocarcinogenesis in HBV genotype C1 infected Cambodians is indicated by 340 HBV C1 full-genomes analysis from GenBank

Approximately 75% of hepatocellular carcinomas (HCC) occur in Asia; core promoter mutations are associated with HCC in HBV genotype C, the dominant genotype in Cambodia. We analyzed these mutations in Cambodian residents and compared them with HBV full genomes registered in GenBank. We investigated...

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Bibliographic Details
Published in:Scientific reports 2019-08, Vol.9 (1), p.12186-11, Article 12186
Main Authors: Chuon, Channarena, Takahashi, Kazuaki, Matsuo, Junko, Katayama, Keiko, Yamamoto, Chikako, Ko, Ko, Hok, Sirany, Nagashima, Shintaro, Akbar, Sheikh Mohammad Fazle, Tanaka, Junko
Format: Article
Language:English
Subjects:
45
45/23
45/70
692/308/174
692/4020/4021/234/2513/1549
Adolescent
Adult
Aged
Aged, 80 and over
Cambodia - epidemiology
Carcinoma, Hepatocellular - etiology
Carcinoma, Hepatocellular - pathology
Child
Cirrhosis
Databases, Genetic
Female
Genomes
Genotype
Genotype & phenotype
Genotypes
Hepatitis
Hepatitis B - complications
Hepatitis B - epidemiology
Hepatitis B - pathology
Hepatitis B - virology
Hepatitis B surface antigen
Hepatitis B Surface Antigens - blood
Hepatitis B virus - classification
Hepatitis B virus - genetics
Hepatitis B virus - isolation & purification
Hepatocellular carcinoma
Humanities and Social Sciences
Humans
Liver cancer
Liver cirrhosis
Liver Neoplasms - etiology
Liver Neoplasms - pathology
Male
Middle Aged
multidisciplinary
Mutation
Phylogeny
Science
Science (multidisciplinary)
Strains (organisms)
Young Adult
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Summary:Approximately 75% of hepatocellular carcinomas (HCC) occur in Asia; core promoter mutations are associated with HCC in HBV genotype C, the dominant genotype in Cambodia. We analyzed these mutations in Cambodian residents and compared them with HBV full genomes registered in GenBank. We investigated the characteristics of 26 full-length HBV genomes among 35 residents positive for hepatitis B surface antigen in Siem Reap province, Cambodia. Genotype C1 was dominant (92.3%, 24/26), with one case of B2 and B4 each. Multiple mutations were confirmed in 24 Cambodian C1 isolates, especially double mutation at A1762T/G1764A in 18 isolates (75.0%), and combination mutation at C1653T and/or T1753V and A1762T/G1764A in 14 isolates (58.3%). In phylogenetic analysis, 16 of 24 isolates were located in the cluster with Laos, Thailand, and Malaysia. In 340 GenBank-registered C1 strains, 113 (33.2%) had combination mutation amongst which 16.5%, 34.2%, and 95.2% were found in ASC, chronic hepatitis, and liver cirrhosis (LC)/HCC respectively ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-48304-z