Failure of complete hatching of ICSI-derived human blastocyst by cell herniation via small slit and insufficient expansion despite ongoing cell proliferation

• Published in: Journal of assisted reproduction and genetics 2019-08, Vol.36 (8), p.1579-1589
• Main Authors: Inoue, Taketo, Uemura, Mikiko, Miyazaki, Kazunori, Yamashita, Yoshiki
• Format: Article
• Language: English
• Subjects: Assisted Reproduction Technologies; Blastocyst - cytology; Blastocyst - physiology; Blastocysts; Cell Proliferation; Cryopreservation; Degeneration; Embryo, Mammalian - cytology; Embryo, Mammalian - physiology; Female; Fertility; Fertilization; Fertilization in Vitro; Gynecology; Hatching; Human Genetics; Humans; In vitro fertilization; Medicine; Medicine & Public Health; Pregnancy; Reproductive Medicine; Risk factors; Sperm Injections, Intracytoplasmic - methods; Time-Lapse Imaging; Trophectoderm; Zona pellucida; Zona Pellucida - physiology
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1007/s10815-019-01521-x

Purpose To assess the effect of intracytoplasmic sperm injection (ICSI) on embryo hatching and visualise the effects of zona thinning (ZT) on the embryo using time-lapse monitoring. Methods In vitro fertilisation (IVF) (n = 178) and ICSI (n = 110)-derived cryopreserved blastocysts were donated by patients who previously had a baby. This study investigated the impacts of IVF, ICSI, laser-assisted hatching by ZT and formation of ICSI penetration trace on zona pellucida of IVF-derived blastocyst on blastcyst diameter, the estimated number of trophectoderm (TE) cells and completed hatching rate. Results The completed hatching rate and diameters of the completely hatched blastocysts at hatching commencement and at the maximum expansion were significantly greater in the IVF than in ICSI groups. The completed hatching rate significantly increased with ZT in both groups. The maximum diameters of the completely hatched blastocysts were significantly smaller in the ZT than in non-ZT groups. The estimated TE cell numbers increased from hatching commencement to their maximum expansion points. The incompletely hatched ICSI-derived blastocysts intermittently herniated cells via small slits until degeneration. The completed hatching rate significantly decreased by the formation of ICSI penetration trace on zona pellucida of IVF-derived blastocyst. Conclusion ICSI-derived blastocysts intermittently release proliferating cells and extracted TE cells and/or inner cell masses via a small slit; thus, blastocyst expansion is not sufficiently increased, leading to a reduced complete hatching rate. Therefore, the ICSI penetration trace potentially has negative effects on blastocyst expansion process in vitro and is a risk factor for the failure of completed hatching.