CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients

The evaluation for surgical resectability of pancreatic ductal adenocarcinoma (PDAC) patients is not only imaging-based but highly subjective. An objective method is urgently needed. We report on the clinical value of a phenotypic circulating tumor cell (CTC)-based blood test for a preoperative prog...

Full description

Saved in:
Bibliographic Details
Published in:EBioMedicine 2019-08, Vol.46, p.133-149
Main Authors: Sun, Yukun, Wu, Guangdong, Cheng, Kok Suen, Chen, Anqi, Neoh, Kuang Hong, Chen, Shuiyu, Tang, Zhewen, Lee, Poh Foong, Dai, Menghua, Han, Ray P.S.
Format: Article
Language:English
Subjects:
Biomarkers, Tumor
Carcinoma, Pancreatic Ductal - diagnosis
Carcinoma, Pancreatic Ductal - mortality
Cell Line, Tumor
CTC phenotyping blood test
Female
Humans
Immunophenotyping
Kaplan-Meier Estimate
Male
Neoplasm Metastasis
Neoplasm Staging
Neoplastic Cells, Circulating - metabolism
Neoplastic Cells, Circulating - pathology
Pancreatic cancer
Pancreatic Neoplasms
Pancreatic Neoplasms - diagnosis
Pancreatic Neoplasms - mortality
PDAC metastatic assessment
PDAC overall survival assessment
Phenotype
Prognosis
Proportional Hazards Models
Reproducibility of Results
Research paper
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The evaluation for surgical resectability of pancreatic ductal adenocarcinoma (PDAC) patients is not only imaging-based but highly subjective. An objective method is urgently needed. We report on the clinical value of a phenotypic circulating tumor cell (CTC)-based blood test for a preoperative prognostic assessment of tumor metastasis and overall survival (OS) of PDAC patients. Venous blood samples from 46 pathologically confirmed PDAC patients were collected prospectively before surgery and immunoassayed using a specially designed TU-chip™. Captured CTCs were differentiated into epithelial (E), mesenchymal and hybrid (H) phenotypes. A further 45 non-neoplastic healthy donors provided blood for cell line validation study and CTC false positive quantification. A validated multivariable model consisting of disjunctively combined CTC phenotypes: “H-CTC≥15.0 CTCs/2ml OR E-CTC≥11.0 CTCs/2ml” generated an optimal prediction of metastasis with a sensitivity of 1.000 (95% CI 0.889–1.000) and specificity of 0.886 (95% CI 0.765–0.972). The adjusted Kaplan-Meier median OS constructed using Cox proportional-hazard models and stratified for E-CTC 
ISSN:2352-3964
2352-3964
DOI:10.1016/j.ebiom.2019.07.044