Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus

Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and dia...

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Bibliographic Details
Published in:Biomedical Journal 2019-06, Vol.42 (3), p.187-192
Main Authors: Hash, Sara, Martinez-Viedma, M Pilar, Fung, Fred, Han, Jee Eun, Yang, Paul, Wong, Charlene, Doraisamy, Loganathan, Menon, Suresh, Lightner, Donald
Format: Article
Language:English
Subjects:
Animal health
Animals
Aquaculture
Bioassays
Biomarkers
Biosensing Techniques - methods
Biosensors
Deoxyribonucleic acid
Diagnostic software
Diagnostic systems
Diarrhea
DNA
Food safety
Gastroenteritis
Humans
Infections
Invertebrates
Laboratories
Magnetic Resonance Imaging - methods
Magnetic Resonance Spectroscopy - methods
Marine environment
Methods
Microbiology
Microorganisms
Molecular biology
Nanoparticles
NMR
Nuclear engineering
Nuclear magnetic resonance
Nuclear safety
Original
Pathogens
Polymerase Chain Reaction - methods
Public health
Quality control
Relaxation time
Seafood
Vibrio parahaemolyticus
Vibrio parahaemolyticus - cytology
Vibrio parahaemolyticus - pathogenicity
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Description
Summary:Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea. A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10  CFU/ml) with Molecular Mirroring using iron nanoparticles coated with target-specific biomarkers capable of binding to DNA of the target microorganism. The NMR system generates a signal (in milliseconds) by measuring NMR spin-spin relaxation time T , which correlates with the amount of microorganism DNA. Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10 -10  CFU/ml) using two DNA extraction methods. In addition, the NMR biosensor system can detect a wide range of microorganism DNAs in different matrices within a short period of time. NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The "hybrid" technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety.
ISSN:2319-4170
2320-2890
DOI:10.1016/j.bj.2019.01.009