Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant ( Solanum melongena L.)

Whole-genome sequences of four EMS (ethyl methanesulfonate)-induced eggplant mutants were analyzed to identify genome-wide mutations. In total, 173.01 GB of paired-end reads were obtained for four EMS-induced mutants and (WT) wild type and 1,076,010 SNPs (single nucleotide polymorphisms) and 183,421...

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Bibliographic Details
Published in:Genes 2019-08, Vol.10 (8), p.595
Main Authors: Xiao, Xi-Ou, Lin, Wenqiu, Li, Ke, Feng, Xuefeng, Jin, Hui, Zou, Huafeng
Format: Article
Language:English
Subjects:
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Anthocyanins
Anthocyanins - biosynthesis
Anthocyanins - genetics
Biosynthesis
Cloning
Cultivars
eggplants
Ethyl methanesulfonate
Ethyl Methanesulfonate - toxicity
Flavonoids
Flavonols
Flavonols - biosynthesis
Flavonols - genetics
Frameshift Mutation
galactosidases
Gene deletion
Gene expression
genes
Genome, Plant
genome-wide association study
Genomes
Genomics
Mutagenesis
Mutagens - toxicity
Mutants
Mutation
Ontology
Point Mutation
Polymorphism
Polymorphism, Single Nucleotide
Single-nucleotide polymorphism
Solanum melongena
Solanum melongena - drug effects
Solanum melongena - genetics
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Summary:Whole-genome sequences of four EMS (ethyl methanesulfonate)-induced eggplant mutants were analyzed to identify genome-wide mutations. In total, 173.01 GB of paired-end reads were obtained for four EMS-induced mutants and (WT) wild type and 1,076,010 SNPs (single nucleotide polymorphisms) and 183,421 indels were identified. The most common mutation type was C/G to T/A transitions followed by A/T to G/C transitions. The mean densities were one SNP per 1.3 to 2.6 Mb. The effect of mutations on gene function was annotated and only 7.2% were determined to be deleterious. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed 10 and 11 genes, which were nonsynonymous mutation or frameshift deletion in 48-5 and L6-5 involved in the anthocyanin biosynthesis or flavone and flavonol biosynthesis. QRT-PCR results showed that only the Sme2.5_06210.1_g00004.1, which was annotated as UFGT (Flavonoid galactosidase transferase), expression significantly decreased in the L6-5 mutant compared with the WT. Also, the Sme2.5_06210.1_g00004.1 expression was lower in the colorless eggplant compared with colorful eggplant in the natural eggplant cultivar. These results suggest that Sme2.5_06210.1_g00004.1 may play a key role in eggplant anthocyanin synthesis.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes10080595