Ratiometric Luminescent Nanoprobes Based on Ruthenium and Terbium-Containing Metallopolymers for Intracellular Oxygen Sensing

A collection of luminescent metal complexes have been widely used as oxygen probes in the biomedical field. However, single intensity-based detection approach usually suffered from errors caused by the signal heterogeneity or fluctuation of the optoelectronic system. In this work, respective rutheni...

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Bibliographic Details
Published in:Polymers 2019-08, Vol.11 (8), p.1290
Main Authors: Zhao, Wu-Xing, Zhou, Chao, Peng, Hong-Shang
Format: Article
Language:English
Subjects:
Aqueous solutions
Biocompatibility
Coordination compounds
Copolymers
Dissolved oxygen
Experiments
Heterogeneity
Lasers
Lifetime
Microscopy
Molecular weight
Nanoparticles
NMR
Nuclear magnetic resonance
Optoelectronics
Oxygen probes
Physiology
Polymers
Ruthenium
Spheroids
Terbium
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Summary:A collection of luminescent metal complexes have been widely used as oxygen probes in the biomedical field. However, single intensity-based detection approach usually suffered from errors caused by the signal heterogeneity or fluctuation of the optoelectronic system. In this work, respective ruthenium (II) and terbium (III) complexes were chosen to coordinate a bipyridine-branched copolymer, so that to produce oxygen-sensitive metallopolymer (Ru-Poly) and oxygen-insensitive metallopolymer (Tb-Poly). Based on the hydrophobic Ru-Poly and Tb-Poly, a ratiometric luminescent oxygen nanoprobe was facilely prepared by a nanoprecipitation method. The nanoprobes have a typical size of ~100 nm in aqueous solution, exhibiting a green-red dual-wavelength emission under the excitation of 300 nm and 460 nm, respectively. The red emission is strongly quenched by dissolved oxygen while the green one is rather stable, and the ratiometric luminescence was well fitted by a linear Stern-Volmer equation. Using the ratiometric biocompatible nanoprobes, the distribution of intracellular oxygen within three-dimensional multi-cellular tumor spheroids was successfully imaged.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym11081290