Effects of Chain Length of Chitosan Oligosaccharides on Solution Properties and Complexation with siRNA

In the context of gene delivery, chitosan has been widely used as a safe and effective polycation to complex DNA, RNA and more recently, siRNA. However, much less attention has been paid to chitosan oligosaccharides (COS) despite their biological properties. This study proposed to carry out a physic...

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Bibliographic Details
Published in:Polymers 2019-07, Vol.11 (8), p.1236
Main Authors: Delas, Tim, Mock-Joubert, Maxime, Faivre, Jimmy, Hofmaier, Mirjam, Sandre, Olivier, Dole, François, Chapel, Jean Paul, Crépet, Agnès, Trombotto, Stéphane, Delair, Thierry, Schatz, Christophe
Format: Article
Language:English
Subjects:
Binding
Biological properties
Biotechnology
Cellulose acetate
Chemical Sciences
Chitosan
Condensed Matter
Degree of polymerization
Deoxyribonucleic acid
DNA
Enthalpy
Life Sciences
NMR
Nuclear magnetic resonance
Oligosaccharides
Parameters
Physics
Polyelectrolytes
Polymerization
Polymers
Pore size
Soft Condensed Matter
Spectrum analysis
Volumetric analysis
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Summary:In the context of gene delivery, chitosan has been widely used as a safe and effective polycation to complex DNA, RNA and more recently, siRNA. However, much less attention has been paid to chitosan oligosaccharides (COS) despite their biological properties. This study proposed to carry out a physicochemical study of COS varying in degree of polymerization (DP) from 5 to 50, both from the point of view of the solution properties and the complexing behavior with siRNA. The main parameters studied as a function of DP were the apparent pK , the solubility versus pH, the binding affinity with siRNA and the colloidal properties of complexes. Some parameters, like the pK or the binding enthalpy with siRNA, showed a marked transition from DP 5 to DP 13, suggesting that electrostatic properties of COS vary considerably in this range of DP. The colloidal properties of siRNA/COS complexes were affected in a different way by the COS chain length. In particular, COS of relatively high DP (≥50) were required to form small complex particles with good stability.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym11081236