Mechanisms of Dendritic Elaboration of Sensory Neurons in Drosophila: Insights from In Vivo Time Lapse

In vivo time-lapse multiphoton microscopy was used to analyze the remodeling of the dendritic arborizing (da) sensory neuron known as dorsal dendritic arborizing neuron E (ddaE) during metamorphosis. After its larval processes have been removed, the cell body of ddaE repositions itself on the body w...

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Bibliographic Details
Published in:The Journal of neuroscience 2004-02, Vol.24 (7), p.1541-1550
Main Authors: Williams, Darren W, Truman, James W
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Cell Differentiation - drug effects
Cell Differentiation - physiology
Dendrites - drug effects
Dendrites - physiology
Development/Plasticity/Repair
Drosophila
Drosophila - drug effects
Drosophila - physiology
Genes, Reporter
Juvenile Hormones - pharmacology
Larva - cytology
Larva - physiology
Metamorphosis, Biological - drug effects
Metamorphosis, Biological - physiology
Microscopy, Fluorescence, Multiphoton
Neurons, Afferent - cytology
Neurons, Afferent - drug effects
Neurons, Afferent - physiology
Pupa - cytology
Pupa - physiology
Pyridines - pharmacology
Time Factors
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Summary:In vivo time-lapse multiphoton microscopy was used to analyze the remodeling of the dendritic arborizing (da) sensory neuron known as dorsal dendritic arborizing neuron E (ddaE) during metamorphosis. After its larval processes have been removed, the cell body of ddaE repositions itself on the body wall between 25 and 40 hr after puparium formation (APF) and begins its adult outgrowth at 40 hr APF. The scaffold of the arbor is laid down between 40 and 54 hr APF, when growth is characterized by high filopodial activity at both terminal and interstitial positions and by branch retraction along with branch establishment. Later in development, filopodial activity remains high but is confined to terminal branches, and branch retraction is no longer seen. Treatment with the insect hormone juvenile hormone (JH), a key regulator of metamorphosis, alters the shape and complexity of the adult dendritic tree in a time-dependent manner. Early treatments with juvenile hormone mimic (JHm) appear to repress extension programs and maintain retraction programs. With later JHm treatments, extension programs appear normal, but retraction programs are maintained beyond their normal time. The JH treatments show the importance of retraction programs in establishing the overall arbor shape.
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.4521-03.2004