A case of mistaken identity: The MAR-1 antibody to mouse FcεRIα cross-reacts with FcγRI and FcγRIV

Because the MAR-1 antibody labeled these cells in the absence of FcεRIα expression, we reasoned it would be more appropriate to call these MAR-1+ moDCs. Because MAR-1+ moDCs have also been reported in mice with viral infection,3 we next tested MAR-1 staining of moDCs in this setting. [...]MAR-1 stai...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2019-04, Vol.143 (4), p.1643-1646.e6
Main Authors: Tang, Xin-Zi, Jung, James B., Allen, Christopher D.C.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity - immunology
Cloning
Cross Reactions - immunology
Dendritic cells
Dendritic Cells - immunology
Flow cytometry
Immune response
Immunization
Immunoglobulins
Inflammation
Leukocytes (basophilic)
Leukocytes (neutrophilic)
Lungs
Macrophages
Mast cells
Mice
Monocytes
Myeloid cells
Neutrophils
Protocol
Receptors, IgE - analysis
Receptors, IgE - immunology
Receptors, IgG - analysis
Receptors, IgG - immunology
Rodents
Software
Staining
Stains & staining
Statistical analysis
Viral infections
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Summary:Because the MAR-1 antibody labeled these cells in the absence of FcεRIα expression, we reasoned it would be more appropriate to call these MAR-1+ moDCs. Because MAR-1+ moDCs have also been reported in mice with viral infection,3 we next tested MAR-1 staining of moDCs in this setting. [...]MAR-1 staining is specific for FcεRIα only on mast cells and basophils, whereas MAR-1 staining on DCs, macrophages, monocytes, and neutrophils is due to FcγR expression. Contrary to prior reports,2-4 our results indicate that the MAR-1+ moDCs observed in mice under inflammatory conditions do not express FcεRI. [...]normal mouse moDCs do not model the FcεRI expression by human DCs in patients with allergic disease. [...]it seems likely that injection of the MAR-1 antibody would cross-link activating FcγRs on these and other myeloid cells, thereby potentially modifying immune responses. [...]we caution investigators in the use of the MAR-1 antibody, and we suggest that in future investigations, FcεRIα KO mice should be used as a critical control.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2018.11.045